滤泡辅助性T细胞相关表达分子在慢性乙型肝炎患者中的表达及意义  

Expression and Significance of Expression Molecule of T Follicular Helper Cells in Patients with Chronic Hepatitis B

作　　者：梁淑慧[1] 黄燕晓[2] 朱晔[1] 时光[3] 庞志宇[1] 黎家希 周迎春[1] Liang Shuhui;Huang Yanxiao;Zhu Ye(The First Affiliated Hospital of Guangzhou Universiry of Traditional Chinese Medicine,Guangdong 510405,China)

机构地区：[1]广州中医药大学第一附属医院检验科,510405 [2]广州中医药大学第一附属医院治未病科,510405 [3]广州中医药大学第一附属医院普内科,510405

出　　处：《医学研究杂志》2020年第11期70-73,共4页Journal of Medical Research

基　　金：广东省科技计划项目(2017ZC0142)。

摘　　要：目的探讨慢性乙型肝炎(CHB)患者外周血Tfh细胞相关表达分子CXCR5、CXCL13、IL-21和TGF-β2的表达水平,分析其在CHB发生、发展中的作用。方法选取2018年10月~2019年3月广州中医药大学第一附属医院收治的CHB患者50例,再选取同期健康体检人员30例作为对照组,采用ELISA法检测两组受试者外周血中CXCR5、CXCL13、IL-21和TGF-β2的表达水平,荧光定量PCR检测HBV DNA,化学发光法检测HBsAg、HBeAg,酶速率法检测AST和ALT等指标。结果CHB患者组CXCR5、CXCL13、IL-21和TGF-β2表达明显高于健康对照组(P<0.01),但与HBV DNA、HBsAg、AST、ALT等之间无相关性(P>0.05)。CHB患者中HBeAg阴性组CXCR5、CXCL13、IL-21和TGF-β2的表达比HBeAg阳性组升高,差异无统计学意义(P>0.05)。结论Tfh细胞及其功能相关分子IL-21和TGF-β2参与了HBV感染后相关的免疫应答反应,可以作为不同免疫状态下免疫损伤的预测指标。Objective To discuss the expression level of chronic-hepatitis-B-related expression molecule CXCR5,CXCL13,IL-21,and TGF-β2 in peripheral blood of patients with chronic hepatitis B(CHB)and to analyze the function of expression molecule in the development of CHB.MethodsThe peripheral blood of 50 patients with CHB in The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were chosen to be compared with the peripheral blood of 30 healthy individuals,controlled group,drawn from the physical examination.Expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in peripheral blood of participating individuals were evaluated through ELISA.HBV DNA of the peripheral blood was tested through PCR.HBsAg and HBeAg were indicated through CLEIA.The levels of AST,ALT,etc.were accessed through continuous monitoring assay.ResultsThe expression levels of CXCR5,CXCL14,IL-21,and TGF-β2 in patients with CHB were significantly higher than the controlled group of healthy individuals(P<0.01),but there was no critical correlation between expression levels and HBV DNA,HBsAg,AST,ALT,etc.(P>0.05).The expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in HBeAg negative group of patients with CHB were slightly higher than the expression levels of CXCR5,CXCL13,IL-21,and TGF-β2 in HBeAg positive group of patients,but the difference of expression level in two groups had no statistical significance(P>0.05).ConclusionFollicular helper T cells,IL-21,and TGF-β2 are associated with the immune response of HBV infection.And they might be used as a predictor of immune damage in different immune states of patients with CHB.

关 键 词：慢性乙型肝炎 滤泡辅助性T细胞 IL-21 TGF-Β2 

分 类 号：R512.6[医药卫生—内科学]