基于网络药理学探讨健脾祛湿化瘀膏对支气管扩张缓解期的作用机制  

Mechanism of Jianpi Qushi Huayu Ointment on the Relief Period of Bronchiectasis Based on Network Pharmacology

作　　者：艾健[1] 王淑英[1] 姬晓辉 冯文杰[1] 齐欢[1] AI Jian;WANG Shuying;JI Xiaohui(Qinhuangdao Hospital of Traditional Chinese Medicine,Hebei,Qinhuangdao,066000,China)

机构地区：[1]秦皇岛市中医医院,河北秦皇岛066000

出　　处：《浙江中医药大学学报》2020年第11期1102-1112,共11页Journal of Zhejiang Chinese Medical University

基　　金：河北省中医药管理局科研计划项目(2020374)。

摘　　要：[目的]分析健脾祛湿化瘀膏的有效化学成分及作用于人体的靶点蛋白,以探索其治疗支气管扩张缓解期的作用机制。[方法]通过中药系统药理学数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)对健脾祛湿化瘀膏的化学活性物质及作用于人体的靶点蛋白进行检索和筛选,同时应用基因组注释数据库平台(genome annotation database platform,GeneCards)预测疾病靶点,绘制关键化学成分-靶点网络图;通过String数据库平台构建蛋白互作(protein-protein interaction,PPI)网络,寻找PPI核心基因,再进行基因本体(gene ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析,找出所涉及的信号通路,构建靶点-信号通路网络图。[结果]通过筛选,共获得健脾祛湿化瘀膏治疗支气管扩张缓解期的关键化学成分107个,共同靶点60个;GO富集分析结果包括营养水平应答、囊泡腔、血红素结合等,KEGG富集分析结果包括晚期糖基化终产物及其受体(advanced glycation end products-receptor for advanced glycation end products,AGE-RAGE)信号通路、肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、白介素-17(interleukin-17,IL-17)信号通路等。[结论]健脾祛湿化瘀膏可能通过调节AGE-RAGE信号通路、TNF信号通路、IL-17信号通路等发挥抗感染、抗炎、调节免疫的功能,进而起到治疗支气管扩张缓解期的作用。[Objective]To analyze the effective chemical components of Jianpi Qushi Huayu ointment and the target protein of the human body in order to explore the mechanism of treatment of the relief period of bronchiectasis.[Methods]The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database was used to search and screen the chemical active substances of Jianpi Qushi Huayu ointment and the target proteins of the human body,and the genome annotation database platform(GeneCards)was used to predict disease target,key chemical component-target network map and protein-protein interaction(PPI)network were constructed through String database platform,PPI core genes were searched,and then gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed to find out the involved signaling pathways and construct the targets-signaling pathway network diagram.[Results]Through screening,a total of 107 key chemical components for the treatment of the relief period of bronchiectasis were obtained,and 60 common targets were obtained;GO enrichment analysis results included response to nutritional levels,vesicle lumen,heme binding,etc.,KEGG enrichment analysis results included advanced glycation end products-receptor for advanced glycation end products(AGE-RAGE)signaling pathway,tumor necrosis factor(TNF)signaling pathway,interleukin-17(IL-17)signaling pathway and so on.[Conclusion]Jianpi Qushi Huayu ointment may play a role in anti-infection,anti-inflammatory,and immune regulation through regulating AGE-RAGE signaling pathway,TNF signaling pathway,IL-17 signaling pathway,etc.to treat the relief period of bronchiectasis.

关 键 词：健脾祛湿化瘀膏 支气管扩张 缓解期 作用机制 调节免疫 网络药理学 中药 

分 类 号：R273[医药卫生—中西医结合]