正常体质-肾虚体质-肾虚证候大鼠腮腺AQP1变化规律研究  

A Study on the Change Rule of Parotid Gland AQP1 in Rats with Normal Constitution-Kidney Deficiency Constitution and Kidney Deficiency Syndrome

作　　者：李翠娟[1] 杨剑 巩振东[1] 孙理军[1] 呼睿 王艺璇 Li Cuijuan;Yang Jian;Gong Zhendong;Sun Lijun;Hu Rui;Wang Yixuan(Shaanxi University of Chinese Medicine,Xianyang Shaanxi,7120462;Shenmu Hospital,Shenmu Shaanxi,719300)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]神木市医院,陕西神木719300

出　　处：《现代中医药》2020年第6期9-14,共6页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81202614);陕西省教育厅重点实验室科研计划项目(17JS032,18JS030);陕西省中医药管理局科研项目(2019-ZZ-JC016);陕西省中医药管理局中医藏象理论重点研究室建设项目(SZDYJS-2);第四批全国中医基础/临床优秀人才研修项目(国中医药人教发[2017]24号);咸阳市中医体质与疾病防治重点实验室重点研发计划项目(2019K01-53);陕西中医药大学中医经典理论研究创新团队建设项目(2019-YL01);2020年陕西中医药大学校级科研课题研究生创新项目(2020CX08)。

摘　　要：目的探讨正常体质-肾虚体质-肾虚证候动态演变过程中大鼠腮腺唾液流量、渗透压、AQP1的变化规律。方法根据中医"恐伤肾"理论,运用猫吓孕鼠方法制作仔鼠肾虚体质动物模型,在此基础上肌注氢化可的松注射液制作肾虚证候动物模型,观察正常对照组、肾虚体质组、肾虚证候组大鼠唾液流量、渗透压及腮腺AQP1的变化。结果 (1)一般状况比较:造模期间,正常对照组大鼠体重增加明显,精神状态良好,皮毛浓密有光泽,饮食正常,大小便良好。与正常对照组比较,肾虚体质组大鼠体重略轻,精神不振,毛发光泽度欠佳,部分大鼠有脱毛现象,摄食量欠佳,大便质软,小便偏多。肾虚证候组大鼠平均体重最轻,毛发稀疏,均有毛发脱落表现,胆小易惊,喜抱团取暖,精神萎靡,活动较少,摄食量少,大便多不成形,小便量多。(2)唾液流量检测:与正常对照组比较,肾虚体质组唾液分泌减少,流量降低,有统计学意义(P<0.05),肾虚证候组唾液流量明显降低,具有显著的统计学意义(P<0.01);(3)唾液渗透压检测:与正常对照组比较,肾虚体质组及肾虚证候组唾液渗透压均较低,具有显著统计学差异(P<0.01);(4)腮腺组织AQP1免疫组化检测:与正常对照组比较,肾虚体质组及肾虚证候组腮腺AQP1表达均减低,具有显著统计学意义(P<0.01);与肾虚体质组比较,肾虚证候组AQP1表达降低,但无统计学差异(P>0.05);(5)腮腺组织AQP1免疫印迹检测:与正常对照组比较,肾虚体质组及肾虚证候组腮腺AQP1表达均减少,具有显著统计学意义(P<0.01);与肾虚体质组比较,肾虚证候组AQP1表达降低,有统计学差异(P<0.05)。结论伴随着"正常体质-肾虚体质-肾虚证候"的动态演变过程,大鼠唾液流量、渗透压及腮腺AQP1表达均发生了变化,而且随着肾虚状态的加重,相应指标的变化更加明显,说明了唾液的分泌确实与肾功能的盛衰密切相关,其发生的内在机Objective To investigate the change rules of parotid saliva flow, osmotic pressure, and AQP1 during the dynamic evolution among rats with normal constitution,kidney deficiency constitution and kidney deficiency syndrome. Methods According to the TCM theory of " kidney injury fear ", an animal model of kidney deficiency in the offspring rats was made by means of cats-scaring-pregnant mice. On this basis, an animal model of kidney deficiency syndrome was made by intramuscular injection of hydrocortisone injection. The normal control group, kidney deficiency constitution and kidney deficiency syndrome were observed in terms of their changes of salivary flow, osmotic pressure and parotid gland AQP1.Results(1)General comparison: During the modeling period, the rats in the normal control group gained significant weight, good mental state, thick and shiny fur, normal diet, and good bowel movements. Compared with the normal control group, the rats in the kidney-deficiency physique group were slightly lighter, weakened,with hair gloss. Some rats had hair loss, poor food intake, soft stools and excessive urination. The rats in the kidney-deficiency syndrome group have the lightest average weight, sparse hair, all have hair loss,and they are timid and easy to be shocked. They like to hug together to keep warm with low spirt less activity, less food intake, more irregular stools, and more urination.(2)Saliva flow test: Compared with the normal control group, the saliva secretion and flow of the kidney deficiency constitution group were decreased, which was statistically significant(P<0.05), and the saliva flow of the kidney deficiency syndrome group was significantly reduced, which was statistically significant(P<0.01).);(3) Saliva osmotic pressure test: Compared with the normal control group, the saliva osmotic pressure of the kidney-deficiency constitution group and the kidney-deficiency syndrome group were lower with significant statistical difference(P<0.01);(4)Parotid gland tissue AQP1 immunohistochemical test:Compared

关 键 词：肾虚体质 肾虚证候 唾液流量 唾液渗透压 AQP1 

分 类 号：R2-03[医药卫生—中医学]