Antihyperglycemic and hepatoprotective properties of miracle fruit(Synsepalum dulcificum) compared to aspartame in alloxan-induced diabetic mice  

作　　者：Suzan G.Haddad Mariam Mohammad Karim Raafat Fatima A.Saleh 

机构地区：[1]Department of Medical Laboratory Sciences.Faculty of Health Sciences,Beirut Arab University,Beirut 115020,Lebanon [2]Department of Pharmaceutical Sciences.Faculty of Pharmacy.Beirut Arab University,Beirut 115020,Lebanon

出　　处：《Journal of Integrative Medicine》2020年第6期514-521,共8页结合医学学报（英文版）

摘　　要：Objective: This study was undertaken to investigate the antihyperglycemic potential of miracle fruit(MF)as well as its hepatic safety as compared to aspartame in alloxan-induced diabetic mice.Methods: MF extracts were prepared and screened for their phytochemical composition using highperformance liquid chromatography(HPLC). Total phenolic, flavonoid and tannin contents and antioxidant potential were also determined. Additionally, MF was evaluated for its sensory attributes. For in vivo work, MF ethanol extract at high(MFH: 500 mg/kg body weight [BW]) and low(MFL: 250 mg/kg BW) doses as well as aspartame were injected intraperitoneally into alloxan-induced diabetic mice.Blood glucose levels were determined following acute and subchronic treatment. At the end of the study,animals were sacrificed, serum was collected for biochemical analysis and liver tissues were obtained for histopathological examination.Results: MF ethanol extract contained more flavonoids and tannins, and had higher 1,1-diphenyl-1-picrylhydrazyl radical-scavenging activity(79.61%) compared to MF aqueous extract(P < 0.05). HPLC analysis of MF ethanol extract also revealed the presence of 10 antioxidants with quercetin comprising the major polyphenol. Additionally, sensory analysis of MF showed that its intake is effective in masking undesirable sourness. Subchronic administration of MFH proved amelioration of hyperglycemia in mice as compared to aspartame. Moreover, aspartame treatment significantly elevated(P < 0.05) the level of alanine aminotransferase and had destructive effects on the liver histopathology;however, hepatic architecture was restored by low and high doses of MF.Conclusion: MF is an effective antihyperglycemic with hepatoprotective properties that can be used as a healthier alternative sweetening agent in place of aspartame for sour beverages.

关 键 词：Miracle fruit Synsepalum dulcificum ASPARTAME Histopathology Phytochemical analysis Sensory evaluation 

分 类 号：R587.1[医药卫生—内分泌]