Effects of co-administration of Ganoderma terpenoid extract with chloroquine on inflammatory markers and antioxidant status in Plasmodium berghei-infected mice  

作　　者：Olarewaju M.Oluba Oghenerobor B.Akpor Feyikemi D.Adebiyi Sunday J.Josiah Olayinka O.Alabi Ayoola O.Shoyombo Augustine O.Olusola 

机构地区：[1]Department of Biochemistry,Food Safety&Toxicology Research Unit,College of Pure&Applied Sciences,Landmark University,Omu-Aran,Kwara State 251101,Nigeria [2]Department of Microbiology,College of Pure&Applied Sciences,Landmark University,Omu-Aran,Kwara State 251101,Nigeria [3]Department of Chemical Sciences,Joseph Ayo Babalola University,Ikeji-Arakeji,Osun State 233121,Nigeria [4]Department of Medical Biochemistry,College of Basic Medical Sciences,Igbinedion University,Okada,Edo State 302110,Nigeria [5]Department of Animal Science,College of Agricultural Sciences,Landmark University,Omu-Aran,Kwara State 251101,Nigeria [6]Department of Biochemistry,Adekunle Ajasin University,Akungba Akoko,Ondo State 342111,Nigeria

出　　处：《Journal of Integrative Medicine》2020年第6期522-529,共8页结合医学学报（英文版）

摘　　要：Objective:To understand the protective effects of Ganoderma terpenoid extract(GTE)against Plasmodium berghei-malarial infection in mice,the present study was carried out to evaluate the effects of GTE in combination with chloroquine disulphate(CQ)on erythrocyte-selected inflammatory markers and antioxidant defense status in P.berghei-infected mice.Methods:P.berghei-infected mice were divided into six groups:infected control(IC)group,administered 1 mL Tween 20;GTE100 and GTE250 groups,administered 100 and 250 mg/kg GTE,respectively;GT100+CQ and GT250+CQ groups,co-administered 100 and 250 mg/kg GTE plus 30 mg/kg CQ,respectively;and CQ group,administered 30 mg/kg CQ.A separate group of non-infected mice were given 1 m L Tween 20,and served as a normal control group(NC).Extract and drug were dissolved in Tween20 and administered orally once daily for 12 consecutive days.At the end of the treatment period,mice were anesthetized with chloroform and sacrificed by cervical dislocation.Plasma was prepared from blood obtained from each mouse.Parameters evaluated at the end of the treatment period include parasitemia,red blood cell count,hematocrit,malondialdehyde(MDA),glutathione(GSH),catalase(CAT),glutathione peroxidase(GPx),superoxide dismutase(SOD),tumor necrosis factor-a(TNF-a),and interleukin-10(IL-10).Results:Infected mice treated with a combination of GTE and CQ(GT100+CQ and GT250+CQ groups)showed significantly reduced parasitemia levels(P<0.05)compared to those administered GTE alone as well as IC.Significant improvement in body weight(P<0.05)was also observed in infected mice treated with a combination of GTE and CQ(GT100+CQ and GT250+CQ groups),compared to mice receiving GTE alone(GTE100 and GTE250 groups).Plasma MDA and TNF-a concentrations were significantly lowered,and IL-10 concentration was significantly increased in GT100+CQ and GT250+CQ groups,relative to the IC group(P<0.05).GSH concentration and SOD,CAT and GPx activities were significantly higher in GT100+CQ and GT250+CQ groups compared to the GTE100,GT

关 键 词：GANODERMA TERPENES MALARIA Oxidative stress Antioxidants Anti-inflammatory agents 

分 类 号：R965[医药卫生—药理学]