猪前交叉韧带重建联合髌骨脱位关节炎模型的分析  

Analysis of osteoarthritis model induced by anterior cruciate ligament reconstruction combined with patella dislocation in a porcine

作　　者：李鹏翠[1] 王春芳 李鹏花[3] 梁斌[3] 胡凯 孙凯[4] 胥毅[4] 卫小春[1] 赵瑞鹏[1] Li Pengcui;Wang Chunfang;Li Penghua;Liang Bin;Hu Kai;Sun Kai;Xu Yi;Wei Xiaochun;Zhao Ruipeng(Department of Orthopaedics,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China;Laboratory Animal Center of Shanxi Medical University,Taiyuan 030002,China;Department of Laboratory Medicine,Fenyang Hospital of The Shanxi Medical University,Fenyang 032200,China;Department of Radiology,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第二医院骨科,太原030001 [2]山西医科大学实验动物中心,太原030002 [3]山西医科大学附属汾阳医院检验科,032200 [4]山西医科大学第二医院影像科,太原030001

出　　处：《中华实验外科杂志》2020年第11期2046-2049,共4页Chinese Journal of Experimental Surgery

基　　金：国家国际科技合作专项项目(2015DFA33050)。

摘　　要：目的探讨小型猪多间室骨关节炎(OA)模型的制备并分析其特征。方法18个月龄健康雌性巴马小型猪6只,购自北京市实创世纪小型猪养殖基地。右后肢(RH)实施前交叉韧带重建联合髌骨脱位术制备多间室OA模型,为实验组。左后肢(LH)不做任何处理,为对照组。术后3个月安乐处死猪,收集LH和RH。离体状态下用X线、CT三维重建、磁共振成像(MRI)方法检查各组膝关节的影像学变化,并采用Kellgren-Lawrenc、全器官磁共振成像(WORM)评分系统评分。计量资料采用t检验或秩和检验;计数资料采用χ2检验。结果X线显示实验组膝关节有少量骨赘形成,偶见狭窄。Kellgren-Lawrence评分显示,对照组0级(n=3),Ⅰ级(n=3);实验组Ⅰ级(n=2),Ⅱ级(n=3),Ⅲ级(n=1),差异无统计学意义(χ2=3.600,P>0.05)。CT三维重建结果显示实验组膝关节面整体粗糙,尤其髌股关节面毛糙。MRI压脂序列像显示实验组膝关节软骨连续性较差。WORM评分显示,实验组滑车内侧关节面(M=2.25,Q1=2.00,Q3=3.25)、内侧髁关节面(M=2.00,Q1=2.00,Q3=2.25)、内侧平台关节面(M=1.50,Q1=1.00,Q3=2.00)、滑车外侧关节面(M=2.25,Q1=1.75,Q3=2.63)、外侧平台关节面(M=1.00,Q1=1.00,Q3=1.63)评分显著高于对照组,差异有统计学意义(P<0.05)。T2值测量显示,实验组滑车内侧关节面(63.94±5.67,t=8.589)、内侧髁关节面(58.02±7.53,t=5.108)、内侧平台关节面(55.82±5.78,t=6.114)、滑车外侧关节面(56.76±8.30,t=7.288)、外侧髁关节面(54.76±3.64,t=25.650)较对照组T2值更大,差异有统计学意义(P<0.05)。结论前交叉韧带重建联合髌骨脱位术可以制备多间室OA模型并明确其影像学特征;MRI对早期OA的评估可能更加敏感、准确。Objective To explore induction of the multi-compartment osteoarthritis modeli and analyze its characteristics.Methods Six healthy female Bama mini pigs at 18 months old were purchased from the Beijing Shichuang Century Minipig Breeding Base.The right hind limb(RH)underwent anterior cruciate ligament reconstruction combined with patella dislocation to induce multi-compartment osteoarthritis,the experiment group.The left hind limb(LH)is not intervened,the control group.The animals were euthanized 3 months after surgery,and LH and RH were collected.X-ray,CT three-dimensional reconstruction and magnetic resonance imaging(MRI)method were used to observe the imaging changes of knee joints in each group.X-ray results were scored using the Kellgren-Lawrenc scoring system and MRI results were scored using the Whole-Organ Magnetic Resonance Imaging(WORM)scoring system.SPSS statistical software was used to analyze the collected data.Measurement data were estimated using t-test or Wilcoxon rank sum test;enumeration data were estimated using chi-square test.Results X-ray showed that the joint space was narrow with a small number of osteophytes,in the experimental group.The Kellgren-Lawrence grade showed no significant difference between the control group(0 grade:n=3;Ⅰgrade:n=3)and the experimental group(Ⅰgrade:n=2;Ⅱgrade:n=3;Ⅲgrade:n=1),(χ2=3.600,P>0.05).CT three-dimensional reconstruction results showed the knee joint surface was rough,especially the patellofemoral joint and a small amount of osteophytes was formed on the lateral and medial side of the femoral condyle,in the experimental group.The MRI OSag-fs PD results showed the knee cartilage was poor in continuity,in the experimental group.WORM score showed medial articular surface of femoral trochlear(M=2.25,Q1=2.00,Q3=3.25),medial femoral condyle(M=2.00,Q1=2.00,Q3=2.25),medial tibial plateau(M=1.50,Q1=1.00,Q3=2.00),lateral articular surface of femoral trochlear(M=2.25,Q1=1.75,Q3=2.63),lateral tibial plateau(M=1.00,Q1=1.00,Q3=1.63)of the experimental group was

关 键 词：骨关节炎 前交叉韧带重建 髌骨脱位 猪 

分 类 号：R684.3[医药卫生—骨科学] R-332[医药卫生—外科学]