一种有机稀土及其与植物甾醇复合物的体外应用评估  

Evaluation of an organic rare earth and its complex with phytosterol in vitro

作　　者：代发文 黄玉岚 于海娜 姚宏明 许甲平 冯一凡 Dai Fawen;Huang Yulan;Yu Haina;Yao Hongming;Xu Jiaping;Feng Yifan

机构地区：[1]乐山师范学院,四川乐山614000 [2]北京大伟嘉生物技术股份有限公司,北京100085 [3]常宁德邦生物科技有限公司,湖南衡阳420500

出　　处：《饲料工业》2020年第23期44-48,共5页Feed Industry

摘　　要：试验旨在应用悬液定量法和猪肠上皮细胞(IPEC-1)培养试验评定一种有机稀土(REE)及其与植物甾醇复合物(REP)的抑菌活性和对细胞增殖的影响。结果发现:①REE和REP对大肠杆菌、沙门氏菌和葡萄球菌抑菌效率随处理浓度(1 mg/ml、500μg/ml、10μg/ml)降低而显著降低(P<0.05),低浓度(10μg/ml)下REP抑菌效率显著低于同浓度REE组(P<0.05)。②与对照组相比,高浓度(1 mg/ml、500μg/ml)REE和REP显著抑制IPEC-1细胞增殖(P<0.05),低浓度(50μg/ml、10μg/ml)REE和REP对IPEC-1增殖均无抑制作用,适宜浓度(50μg/ml)REP对IPEC-1还表现出显著的促生长作用(P<0.05)。由此可见,高浓度有机稀土及其与植物甾醇复合物具有较好体外抑菌效率,显著抑制IPEC-1细胞增殖,低浓度下添加植物甾醇的REP抑菌效率降低并促进IPEC-1细胞增殖。The objective of the current study was to investigate the antibacterial efficiency of organic rare earth elements(REE)and its complex with phytosterol(REP)with the suspension quantitative method,and their effects on cell proliferation with pig intestinal epithelial cells(IPEC-1).The results showed that:①the antibacterial efficiency of REE and REP on Escherichia coli,Salmonella and Staphylococcus decreased significantly with the decreasing of treatment concentration(1 mg/ml,500μg/ml and 10μg/ml)(P<0.05),and the antibacterial efficiency of REP at the low concentration(10μg/ml)was significantly lower than that of REE with the same concentration(P<0.05).②REE and REP at high concentration(1 mg/ml and 500μg/ml)had significant inhibition effect on the growth of IPEC-1 compared with those of control group(P<0.05),while it did not show inhibition difference at low concentration group(50μg/ml and 10μg/ml),and REP at the appropriate concentration(50μg/ml)showed significant growing-promoting effect on IPEC-1(P<0.05).These results showed that both of REE and REP at high concentration have good antibacterial efficiency and significant inhibitory effect on cell proliferation of IPEC-1.REP with phytosterol at low concentration can relieve the antibacterial effect and promote cell proliferation.

关 键 词：有机稀土 植物甾醇 抑菌效率 肠上皮细胞 细胞增殖 

分 类 号：S816.7[农业科学—饲料科学]