紫苏油脂合成相关基因家族鉴定及表达分析  

Identification and Expression Analysis of Gene Family Related to Perilla Lipid Synthesis

作　　者：郝月茹 周雅莉 王志龙 薛应红 邢志 李润植[1] 王计平[1] HAO Yueru;ZHOU Yali;WANG Zhilong;XUE Yinghong;XING Zhi;LI Runzhi;WANG Jiping(Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China)

机构地区：[1]山西农业大学分子农业与生物能源研究所,山西太谷030801

出　　处：《西北植物学报》2020年第10期1663-1671,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山西省重点研发计划(201803D221005-9);国家青年科学基金(31201266);山西省晋中市科技重点研发计划(Y172007-5)。

摘　　要：该研究从转录组数据库中筛选鉴定紫苏溶血磷脂酸酰基转移酶(LPAT)家族基因,采用生物信息学方法分析了该家族基因的序列特征及蛋白结构,利用qRT-PCR技术对该基因时空表达特性进行了研究,为进一步了解紫苏油脂合成机制提供理论依据。结果表明:(1)从紫苏转录组数据库中共检测出11个LPAT家族基因,分别命名为PfLPAT1、PfLPAT2-1、PfLPAT2-2、PfLPAT2-3、PfLPAT2-4、PfLPAT4-1、PfLPAT4-2、PfLPAT5-1、PfLPAT5-2、PfLPAT5-3和PfLPAT5-4;PfLPATs编码氨基酸长度介于250~384 aa之间,理论等电点在7.6~9.6之间。(2)基因序列比对结果表明,11个PfLPATs蛋白分别属于3个亚类,其中1型LPAT包含1个基因,2/3型LPAT包含4个,4/5型LPAT包含6个。(3)实时荧光定量PCR结果显示,11个LPAT家族基因在‘晋紫苏1号’不同组织中均有表达,其中LPAT2-1、LPAT2-2和LPAT2-3在种子中表达量较高,推测其在紫苏种子油脂合成代谢过程中发挥重要作用。该结果为后续紫苏LPAT家族基因的功能研究提供了重要的基因信息。The lysophosphatidic acid acyltransferase(LPAT)family genes were screened and identified from the transcriptome database of perilla.The sequence characteristics and protein structures of the family genes were analyzed using bioinformatics methods and the spatio-temporal expression characteristics of genes were studied using qRT-PCR technology.The research provides a basis for better understanding the mechanism of perilla oil synthesis.The results showed that:(1)a total of 11 LPAT family genes were detected,including PfLPAT1,PfLPAT2-1,PfLPAT2-2,PfLPAT2-3,PfLPAT2-4,PfLPAT4-1,PfLPAT4-2,PfLPAT5-1,PfLPAT5-2,PfLPAT5-3 and PfLPAT5-4.The length of amino acids encoded by PfLPATs genes ranged from 250 to 384 aa,and the theoretical isoelectric point varied from 7.6 to 9.6.(2)The analysis of gene sequence comparison showed that 11 PfLPATs proteins belonged to three subclasses,respectively,among which type 1 LPAT contained one gene,type 2/3 LPAT contained four genes and type 4/5 LPAT contained six genes.(3)Real-time fluorescent quantitative PCR results indicated that 11 LPAT family genes were expressed in different tissues of‘Jin Zisu 1’.Among them,LPAT2-1,LPAT2-2 and LPAT2-3 expressed highly in seeds.It was speculated that they play an key role in the process of perilla seed oil synthesis and metabolism.The results of this study provided important genetic information for further study on the functions of LPAT family genes.

关 键 词：紫苏 LPAT家族基因 油脂代谢 基因序列特征 表达特性 

分 类 号：Q785[生物学—分子生物学] Q789