多胺对盐胁迫下黄瓜SOS2基因家族表达的影响  

Effect of Polyamines on SOS2 Family Gene Expression in Cucumber under Salt Stress

作　　者：王丹怡 韩玲娟 张毅[1] 张莉[1] 刘东旭 李斌 WANG Danyi;HAN Lingjuan;ZHANG Yi;ZHANG Li;LIU Dongxu;LI Bin(Collaborative Innovation Center for Improving Quality and Increase Profits of Protected Vegetables, College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China;College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China)

机构地区：[1]山西农业大学园艺学院,山西省设施蔬菜提质增效协同创新中心,山西太谷030801 [2]山西农业大学草业学院,山西太谷030801

出　　处：《西北植物学报》2020年第11期1855-1865,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31501806);山西农业大学博士科研启动项目(2014YJ14,2020BQ20);山西省重点研发计划重点项目子课题(201703D211001-04-03)。

摘　　要：该研究基于黄瓜基因组数据库,利用生物信息学和荧光定量PCR等方法,对黄瓜SOS2基因家族进行全基因组鉴定,并对其表达特性进行系统分析。结果表明:(1)在黄瓜基因组中,共鉴定到15个SOS2基因(CsSOS2-1~CsSOS2-15),且不均匀分布在5条染色体上;亚细胞定位显示SOS2蛋白主要位于细胞质膜。(2)系统进化分析显示,CsSOS2-2和CsSOS2-6与AtSOS2亲缘关系更近。(3)顺式作用元件预测显示,SOS2基因启动子序列主要含有干旱诱导和防御应激响应元件。(4)结构分析显示,SOS2蛋白所含保守基序的排列顺序完全一致,且主要含有STKc和NAF保守结构域,这些结构可能在基因响应盐胁迫过程中起调控作用。(5)荧光定量试验表明,SOS2基因家族主要在黄瓜的根和叶片响应盐胁迫时上调表达,其中,盐胁迫处理1 d时有5个基因上调表达,并随处理时间延长盐胁迫下的基因表达有所下调;增施多胺显著上调了CsSOS2-1~CsSOS2-5、CsSOS2-8、CsSOS2-9、CsSOS2-12和CsSOS2-15在不同组织中的表达,说明盐胁迫下多胺能诱导黄瓜SOS2基因家族的表达,进而参与植物耐盐分子网络的调控。The SOS signal transduction pathway is a salt stress signal response pathway in plants,and serine/threonine protein kinase(SOS2)plays an important role in this pathway.Based on the cucumber genome database,this study used bioinformatics and quantitative real-time PCR and other methods to identify the whole genome of cucumber SOS2 gene family,and systematically analyzed its expression characteristics.The results showed that:(1)a total of 15 SOS2 genes(CsSOS2-1-CsSOS2-15)were identified in the cucumber genome,which were distributed unevenly on 5 chromosomes,and the subcellular localization showed that SOS2 protein were mainly located in the plasma membrane.(2)Phylogenetic analysis showed that CsSOS2-2 and CsSOS2-6 were more closely related to AtSOS2.(3)The prediction of cis-acting elements shows that the promoter sequence of SOS2 gene mainly contains drought-induced and defense-stress response elements.(4)Structural analysis showed that the sequence of conserved motifs contained in SOS2 protein were completely consistent,and mainly contained STKc and NAF conserved domains,which may play a regulatory role in gene response to salt stress.(5)Quantitative real-time PCR test showed that the SOS2 gene family is mainly upregulated in cucumber roots and leaves in response to salt stress.Among them,6 genes were up-regulated in response to salt stress on the first day of treatment.As the treatment time prolonged,gene expression was down-regulated under salt stress.Addition of polyamines significantly increased the expression of CsSOS2-1—CsSOS2-5,CsSOS2-8,CsSOS2-9,CsSOS2-12 and CsSOS2-15 in different tissues.This shows that polyamines can induce the expression of cucumber SOS2 gene family under salt stress,and then participate in the regulation of plant salt tolerance molecular network.

关 键 词：黄瓜 SOS2基因家族 盐胁迫 多胺 

分 类 号：Q786[生物学—分子生物学] S642.2[农业科学—蔬菜学]