冻伤蓝变对广义虾脊兰属植物中4种吲哚基衍生物含量的影响  

Effect of Freezing Blue-changing on the Contents of Four Indolyl Derivatives in the Calanthe s.l.

作　　者：张一鸣 万会花[2] 苏勇 鲁梦 杨维 翟俊文[1,3] ZHANG Yiming;WAN Huihua;SU Yong;LU Meng;YANG Wei;ZHAI Junwen(College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;3Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Fuzhou 350002, China)

机构地区：[1]福建农林大学园林学院,福州350002 [2]中国中医科学院中药研究所,北京100700 [3]兰科植物保护与利用国家林业与草原局重点实验室,福州350002

出　　处：《西北植物学报》2020年第11期1951-1958,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建农林大学博士生导师培育项目(YSYL-bdpy-2);中央级公益性科研院所基本科研业务费专项(ZZ11-096)。

摘　　要：以6种广义虾脊兰属植物和2种树兰亚科植物为材料,利用液相色谱-串联三重四极杆质谱仪(LCMS-QQQ)测定了冻伤处理前后花和叶片中靛苷、靛红、靛蓝和靛玉红4种吲哚基衍生物的含量,分析广义虾脊兰属植物吲哚基衍生物的生成及种属间含量的差异。结果显示:(1)4种吲哚基衍生物在所测定的6种广义虾脊兰属植物中均被检出,但在2种树兰亚科植物五唇兰和足茎毛兰中均未被发现。(2)在所测定的6种广义虾脊兰属植物花和叶片中,冻伤处理后的靛蓝、靛玉红和靛红含量均显著上升,而靛苷含量显著下降,同时花中的吲哚基衍生物含量均高于叶片。(3)6种广义虾脊兰属植物花和叶中吲哚基衍生物总含量以黄兰花最高,三褶虾脊兰叶最低。研究表明,冻伤处理引起靛苷向靛蓝的大量转化是导致冻伤后广义虾脊兰属植物组织中呈现出蓝色的主要原因,推测吲哚基衍生物可能也是一类与植物防御相关的化合物,在植物抵御逆境中扮演着重要的角色。Using 6 species of Calanthe s.l.plants and 2 kinds of Epiendroidea plants as materials,we determined indican,isatin,indigo and indirubin contents in flowers and leaves before and after freezing treatment with liquid chromatography-tandem triple quadrupole mass spectrometer(LCMS-QQQ).Analyzed the content of indoxyl derivatives of Calanthe s.l.plants and the differences between species.The results showed that:(1)these 4 indoxyl derivatives were detected in all the 6 Calanthe s.l.plants,but not in the 2 Epidendroidea plants Doritis pulcherrima and Eria coronaria.(2)In the flowers and leaves of 6 Calanthe s.l.plants,the contents of indigo,indirubin and isatin increased significantly after freezing treatment,while the content of indican decreased significantly.At the same time,the content of indoxyl derivatives in flowers was higher than that in leaves.(3)The total content of indoxyl derivatives in the flowers and leaves of the 6 Calanthe s.l.plants were the highest in Cephalantheropsis gracilis flowers and the lowest in Calanthe triplicata leaves.This work has shown that the massive conversion of indican to indigo caused by freezing treatment is the main reason for the blue color in the tissues of the Calanthe s.l.after freezing.It is speculated that indoxyl derivatives may also be a class of compounds related to plant defense and play an important role in plant resistance to adversity.

关 键 词：广义虾脊兰属 吲哚基衍生物 冻伤 蓝变 

分 类 号：Q945.79[生物学—植物学] Q946-33