原人参二醇对非小细胞肺癌的抑制作用及机制  

Inhibitory effect and mechanism of protopanaxdiol on non-small cell lung cancer

作　　者：李秋妍 王浩丞 彭瑶 任德志 朱文斌 王小龙 于海涛 张春晶[1] LI Qiu-yan;WANG Hao-cheng;PENG Yao;REN De-zhi;ZHU Wen-bin;WANG Xiao-long;YU Hai-tao;ZHANG Chun-jing(School of Medical Technology,Qiqihar Medical University,Qiqihar 161006,Heilongjiang Province,China;Basic Medical School,Jiamusi University,Jiamusi 154000,Heilongjiang Province,China;Department of Neurosurgery,Qiqihar First Hospital,Qiqihar 161000,Heilongjiang Province,China)

机构地区：[1]齐齐哈尔医学院医学技术学院,黑龙江齐齐哈尔161006 [2]佳木斯大学基础医学院,黑龙江佳木斯154000 [3]齐齐哈尔第一医院神经外科,黑龙江齐齐哈尔161000

出　　处：《中国临床药理学杂志》2020年第22期3657-3660,共4页The Chinese Journal of Clinical Pharmacology

基　　金：黑龙江省教育厅基本科研业务费基础研究基金资助项目(2017-QYKYYWF-0754);黑龙江省博士后管理办公室博士后研究人员落户黑龙江科研启动资助金基金资助项目(LBH-Q17177)。

摘　　要：目的研究人参活性成分原人参二醇(Protopanaxdiol,PPD)对非小细胞肺癌的抑制作用及机制。方法将人非小细胞肺癌A549细胞分为对照组(等量二甲基亚砜DMSO)和实验组(30μg·mL-1 PPD)。用JC-1标记的流式细胞术检测细胞线粒体膜电位,用激光共聚焦显微镜观察线粒体通透性转换孔道(mitochondrial permeability transition pore,mPTP)变化,用Western blot实验检测解偶连蛋白-2(Uncoupling protein 2,UCP2)的表达水平。结果实验组和对照组红绿荧光的相对比例分别为0.29±0.05和2.44±0.03,钙黄绿素的荧光强度分别为2.79±0.25和8.58±1.83,UCP2蛋白相对表达量分别为0.44±0.03和1.00±0.15,差异均有统计学意义(均P<0.05)。结论 PPD可诱导人非小细胞肺癌A549细胞凋亡,其机制可能与抑制UCP2的基因表达和调节线粒体功能相关。Objective To explore the inhibitory effect and mechanism of protopanaxadiol, an active ingredient of ginseng, on non-small cell lung cancer. Methods Non-small cell lung cancer A549 cell line was divided into two groups: control group(equal dimethyl sulfoxide DMSO) and test group(PPD dilution 30 μg·mL-1). JC-1 labeled flow cytometry was used to detect cell mitochondrial membrane potential, laser confocal microscope was used to observe the changes of mitochondrial permeability transition pore(mPTP), Western blot experiment detected the expression level of uncoupling protein 2(UCP2). Results In the test group and control group, the relative ratio of red-green fluorescence was 0.29±0.05 and 2.44±0.03, the fluorescence intensity of calcein was 2.79±0.25 and 8.58±1.83, the relative expression of UCP2 protein was 0.44±0.03 and 1.00±0.15,the differences of the factors were significant(all P<0.05). Conclusion PPD can inhibit the proliferation and induce apoptosis of human lung adenocarcinoma A549 cells, and its mechanism may be related to inhibiting UCP2 gene expression and regulating mitochondrial function.

关 键 词：原人参二醇 解偶联蛋白2 A549细胞 线粒体损伤 

分 类 号：R28[医药卫生—中药学]