沉香醇提物对顺铂诱导家鸽呕吐的止呕作用研究  

Anti-vomiting Effect of Agarwood Alcohol Extract on Vomiting Pigeon Model Induced by Cisplatin

作　　者：王灿红 彭德乾[2] 刘洋洋[1] 余章昕 郭鹏[1,3] 魏建和 WANG Can-hong;PENG De-qian;LIU Yang-yang;YU Zhang-xin;GUO Peng;WEI Jian-he(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine&Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch Institute of Medicinal Plant,Chinese Academy of Medical Sciences&Peking Union Medical College,Haikou 570311,China;School of Pharmacy,Hainan Medical College,Haikou 571199,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Science&Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室,海南海口570311 [2]海南医学院药学院,海南海口571199 [3]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室,北京100193

出　　处：《中国现代中药》2020年第10期1611-1617,共7页Modern Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1706400);海南省自然科学基金项目(817290,217291);中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-003);海南省自然科学基金创新团队项目(2017CXTD022)。

摘　　要：目的:探讨沉香醇提物对顺铂(DDP)致家鸽呕吐的止呕作用。方法:沉香各醇提物预处理给药后,采用DDP腹腔注射制备家鸽呕吐模型,记录并分别计算呕吐潜伏期、呕吐频率和0~2、2~4、4~6 h呕吐次数,观察家鸽胃腺组织的病理损伤情况,酶联免疫吸附(ELISA)法检测血清中致呕神经递质5-羟色胺(5-HT)和多巴胺(DA)的分泌水平。结果:与模型组比较,沉香醇提物能显著延长呕吐家鸽的呕吐潜伏期,减少呕吐次数和呕吐频率,缓解胃腺组织的病理损伤程度,降低血清中5-HT和DA的分泌水平。结论:沉香醇提物可显著抑制DDP所致的家鸽呕吐,其作用机制可能与调节神经递质有关。Objective:This study aims to investigate the anti-emetic effect of agarwood alcohol extract on vomiting model induced by cisplatin(DDP)in pigeon.Methods:Pigeon was pretreated with agarwood alcohol extract.Pigeon vomiting model was produced by intraperitoneal injection of DDP.Vomiting incubation period,vomiting frequency,and 0-2,2-4 and 4-6 h vomiting numbers of pigeon were recorded and calculated,and observed the pathological damage of pigeon gastric tissues.The secretion levels of serotonin(5-HT)and dopamine(DA)were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the model group,agarwood alcohol extract produced via the whole-tree agarwood-inducing technique(WTAAE)significantly extended the vomiting incubation period,reduced the vomiting frequency and vomiting numbers,relieved the pathological damage of gastric gland tissue,and decreased the secretion levels of 5-HT and DA in serum.Conclusion:Agarwood alcohol extract show significantly anti-vomiting effect on vomiting model in pigeon,and its mechanism may be related to the regulation of neurotransmitter.

关 键 词：沉香醇提物 顺铂 呕吐 止呕作用 神经递质 

分 类 号：R285.5[医药卫生—中药学]