新型络合试剂-紫外分光光度法测定石膏中钙含量  

Determination of Calcium in Gypsum Fibrosum by Novel Complexometry Reagent Coupled with Ultraviolet Visible Spectrophotometry

作　　者：李晓明[1] 魏旭雅 赖长江生[1] LI Xiao-ming;WEI Xu-ya;LAI Chang-jiang-sheng(State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药资源中心道地药材国家重点实验室,北京100700

出　　处：《中国现代中药》2020年第10期1714-1718,共5页Modern Chinese Medicine

基　　金：国家药品标准提高暨2015版药典矿物药项目(ZZ2014011);中国科协青年人才托举工程(CAST-2018-QNRC1-04);中央级公益性科研院所基本科研业务费专项资金资助(ZZ13-YQ-090)。

摘　　要：目的:建立新型络合试剂-紫外分光光度法对石膏中含水硫酸钙进行含量测定的方法。方法:利用新型络合试剂乙二醛双缩(2-羟基苯胺)(GBHA)溶液,在碱性条件下与钙离子生成稳定的络合物,用比色法测定。结果:测定了18批生石膏样品的含量,检测质量浓度为0~15.0μg·mL^-1与吸光度线性关系良好,Y=0.0433 X+1.785×10-3(r=0.9992),平均加样回收率为101%(RSD为0.10%,n=6)。结论:新型络合试剂-紫外可见分光光度法测得样品中钙的含量准确,可以作为石膏类含钙矿物药的含量测定替代方法。Objective:To establish a novel complexometry reagent-UV-spectrophotometric method for content determination of calcium sulfate dihydrate in Gypsum Fibrosum.Methods:Calcium ions may be determined by forming a stable complex within the solution of the glyoxal-bis-(2-hydroxyanil)(GBHA),a novel complexometry reagent,under alkaline conditions.Results:A total of 18 batches of Gypsum Fibrosum were determined.The average recovery was 101% with the RSD of 0.10% for 6 replicates and the equations of linear calibration curve was Y=0.0433 X+1.785×10-3(r=0.9992)under the concentration range of 0-15.0μg·mL^-1.Conclusion:The method established may be alternative and can be used for the determination of calcium sulphate dihydrate in Gypsum Fibrosum.

关 键 词：络合试剂 紫外-可见分光光度法 石膏 含水硫酸钙 含量测定 

分 类 号：R282.76[医药卫生—中药学]