支气管哮喘患者血清肿瘤坏死因子-α、白细胞介素-8和嗜酸性粒细胞阳离子蛋白水平变化及意义  

Changes and significance of serum tumor necrosis factor-α,interleukin-8 and eosinophil cationic protein levels in patients with bronchial asthma

作　　者：庄鹏晖[1] 刘亮亮[2] 郑国玺[3] 潘小明[1] 尚东[4] ZHUANG Penghui;LIU Liangliang;ZHENG Guoxi(Department of Surgery,Xi’an Jiaotong University Hospital,Xi’an 710049)

机构地区：[1]西安交通大学医院外科,西安710049 [2]西安交通大学医院耳鼻咽喉科,西安710049 [3]西安交通大学第二附属医院耳鼻咽喉头颈外科病院,西安710004 [4]西安交通大学第一附属医院呼吸内科,西安710061

出　　处：《陕西医学杂志》2020年第12期1597-1599,1603,共4页Shaanxi Medical Journal

基　　金：陕西省重点研发计划项目(2020GXLH-Y-017)。

摘　　要：目的:探讨支气管哮喘(BA)患者血清肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)和嗜酸性粒细胞阳离子蛋白(ECP)的变化及其意义。方法:收集170例BA患者作为病例组,根据发作情况分为急性发作期组与临床缓解期组,每组85例。同时选择150例健康体检者作为对照组。采用酶联免疫吸附测定法(ELISA)检测各组TNF-α、IL-8和ECP水平。测定第1秒用力呼气容积(FEV 1)占预计肺活量百分比(FEV 1%pred)和FEV 1占用力肺活量比值(FEV 1/FVC)。分析TNF-α、IL-8和ECP间及其与肺功能指标FEV 1%pred和FEV 1/FVC的相关性。结果:急性发作期组和临床缓解期组TNF-α、IL-8、ECP水平均高于对照组,急性发作期组上述指标又高于临床缓解期组,差异均有统计学意义(P<0.05)。各组FEV 1%pred、FEV 1/FVC比较,差异均有统计学意义(P<0.05)。BA患者血清TNF-α、IL-8、ECP水平间均呈正相关关系,而与FEV 1%pred和FEV 1/FVC呈负相关关系。结论:血清TNF-α、IL-8和ECP升高与BA的发生及发展密切相关,进一步了解其网络作用机制将对免疫治疗及开发新的治疗靶点和方法提供新思路。Objective:To investigate the changes and significance of serum tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and eosinophil cationic protein(ECP)in patients with bronchial asthma(BA).Methods:A total of 170 BA patients were collected as a case group,and divided into acute attack group and clinical remission group according to their onset status,with 85 cases in each group.At the same time,150 healthy subjects were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of TNF-α,IL-8 and ECP in each group.The FEV 1%pred and FEV 1/FVC were determined.Analyze The correlation between TNF-α,IL-8 and ECP,and the correlation between them and FEV 1%pred and FEV 1/FVC was analyzed.Results:The levels of TNF-α,IL-8 and ECP in the acute attack group and the clinical remission group were higher than those in the control group(P<0.05).The above indicators in the acute attack group were higher than those in the clinical remission group(P<0.05).The differences of FEV 1%pred and FEV 1/FVC in each group were statistically significant(P<0.05).The serum TNF-α,IL-8 and ECP levels of BA patients were all positively correlated,but negatively correlated with FEV 1%pred and FEV 1/FVC.Conclusion:The elevation of serum TNF-α,IL-8 and ECP is closely related to the occurrence and development of BA.Further understanding of its network mechanism will provide new ideas for immunotherapy and the development of new therapeutic targets and methods.

关 键 词：支气管哮喘 肿瘤坏死因子-α 白细胞介素-8 嗜酸性粒细胞阳离子蛋白 酶联免疫吸附法 第1秒用力呼气容积 

分 类 号：R562.2[医药卫生—呼吸系统]