芦丁减轻全氟辛酸暴露致小鼠心肌损伤的作用研究  

Effect of rutin on reducing myocardial injury induced by perfluorooctanoic acid exposure in mice

作　　者：程静静 余丽丽 周勇兵 汤雨婷 马雨妍 吴俊英[2,4] 刘辉 CHENG Jing-jing;YU Li-li;ZHOU Yong-bing;TANG Yu-ting;MA Yu-yan;WU Jun-ying;LIU Hui(School of Laboratory Medicine,Bengbu Medical College,Bengbu Anhui 233030,China;Clinical Laboratory Diagnostics Experimental Training Center,Bengbu Medical College,Bengbu Anhui 233030,China;School of Public Health,Bengbu Medical College,Bengbu Anhui 233030,China;School of Basic Medicine,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院检验医学院,安徽蚌埠233030 [2]蚌埠医学院临床检验诊断学实验实训中心,安徽蚌埠233030 [3]蚌埠医学院公共卫生学院,安徽蚌埠233030 [4]蚌埠医学院基础学医学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2020年第11期1462-1466,共5页Journal of Bengbu Medical College

基　　金：安徽省高校自然科学研究重点项目(KJ2019A0315);蚌埠医学院自然科学研究重点项目(BYKY1705ZD);国家级大学生创新训练计划(201910367029);安徽省大学生创新训练计划(S201910367052)。

摘　　要：目的:探讨芦丁对全氟辛酸(PFOA)造成的小鼠心肌损伤的保护作用研究。方法:采用PFOA暴露建立小鼠心肌损伤模型,观察芦丁对PFOA造成小鼠心肌损伤模型体质量、心脏质量、心肌TG、TC含量和血清LDH活性、氧化应激指标变化(心肌MDA含量、GSH-PX和T-SOD活性)及心肌组织形态学变化。结果:各组小鼠体质量1~2 d比较差异无统计学意义(P>0.05);3~14 d各组小鼠体质量有差异,且PFOA组小鼠体质量低于PFOA+芦丁组(P<0.05);PFOA组和PFOA+芦丁组小鼠干预14 d心脏质量低于对照组,而PFOA+芦丁组高于PFOA组(P<0.05)。PFOA组小鼠14 d心肌TC含量及血清LDH活性高于对照组(P<0.05);PFOA+芦丁组小鼠14 d心肌TG含量及血清LDH活性低于PFOA组(P<0.05);而PFOA组与PFOA+芦丁组小鼠14 d心肌TC含量差异无统计学意义(P>0.05);PFOA组小鼠14 d心肌MDA含量高于对照组(P<0.05),而PFOA组与对照组小鼠14 d心肌T-SOD、GSH-PX活性差异无统计学意义(P>0.05);PFOA+芦丁组小鼠14 d心肌GSH-PX活性高于PFOA组,而PFOA+芦丁组与PFOA组在小鼠14 d时心肌MDA含量、GSH-PX活性差异无统计学意义(P>0.05)。结论:芦丁干预可以减轻PFOA暴露致小鼠心肌损伤作用。Objective:To explore the protective effects of rutin on myocardial injury induced by perfluorooctanoic acid(PFOA)exposure in mice.Methods:PFOA was used to establish a model of myocardial injury in mice,and the effects of rutin on the body weight,heart weight,myocardial TG,TC content,serum LDH activity,and oxidative stress indicators(myocardial MDA content,GSH-PX and T-SOD activity)and myocardial morphology were investigated in mice.Results:There was no statistical significance in the weight of 1-2 d mice among all groups(P>0.05).The differences of in the weight of 3-14 d mice on day 8 to day 14 among all groups were statistically significant(P<0.05),and the weight of mice in PFOA group was lower than that in PFOA+rutin group,the heart weight of mice in PFOA group and PFOA+rutin group was lower than that in control group after 14 days of intervention,while the heart weight of mice in PFOA+rutin group was higher than that in PFOA group(P<0.05).After 14 days of intervention,the myocardial TC content and serum LDH activity in PFOA group were higher than those in control group(P<0.05),the myocardial TG content and serum LDH activity in PFOA+rutin group were lower than those in PFOA group(P<0.05),and the difference of the myocardial TC content between PFOA group and PFOA+rutin group was not statistically significant(P>0.05).After 14 days of inverention,the myocardial MDA content and in PFOA group was higher than those in control group(P<0.05),but there was no statistical significance in myocardial GSH-PX activity between PFOA group and the control group(P>0.05),the myocardial MDA content in PFOA+rutin group was lower than that in PFOA group,while the differences of the myocardial MDA content and GSH-PX activity between PFOA+rutin group and PFOA group were not statistically significant(P>0.05).Conclusions:Rutin intervention can ameliorate the myocardial injury induced by PFOA exposure in mice.

关 键 词：心肌损伤 芦丁 全氟辛酸 毒理 

分 类 号：R542.2[医药卫生—心血管疾病]