8-isoprostane、SOD、AOA、NF-κB及iNOS在复发性流产病人中的表达研究  

Study on the expression levels of 8-isoprostane,SOD,AOA,NF-κB and iNOS in patients with recurrent spontaneous abortion

作　　者：汪莉[1] 石慧芬 江海英[1] 刘金霞 陈茵[1] WANG Li;SHI Hui-fen;JIANG Hai-ying;LIU Jin-xia;CHEN Yin(Department of Gynaecology,Nanhai Hospital,Guangdong People′s Hospital,The Second People′s Hospital of Nanhai District of Foshan,Foshan Guangdong 528251,China)

机构地区：[1]广东省人民医院南海医院(佛山市南海区第二人民医院)妇科,528251

出　　处：《蚌埠医学院学报》2020年第11期1512-1514,1517,共4页Journal of Bengbu Medical College

基　　金：广东省佛山市科技创新项目(2017AB000102)。

摘　　要：目的:分析8-异构前列腺素(8-isoprostane)、超氧化物歧化酶(SOD)、血浆总抗氧化能力(AOA)、转录因子-κB(NF-κB)和诱导型一氧化氮合酶(iNOS)在复发性流产病人中的表达及影响,以探讨不明原因复发性流产发病机制。方法:选取2018年1月至2019年10月119例产妇作为研究对象,分为不明原因复发性流产病人60例作为研究对象(病例组),进行正常妊娠分娩妇女作为参照对象59例(对照组),比较2组病人血液和绒毛蜕膜组织中的8-isoprostane、SOD、AOA等氧化应激指标及NF-κB、iNOS水平。结果:2组病人血液中AOA水平差异无统计学意义(P>0.05),病例组病人血液中8-isoprostane、NF-κB和iNOS水平均高于对照组(P<0.01),SOD水平明显低于对照组(P<0.01);2组病人绒毛蜕膜组织中AOA水平差异无统计学意义(P>0.05),病例组绒毛蜕膜组织中8-isoprostane、NF-κB和iNOS水平均高于对照组(P<0.01),SOD水平明显低于对照组(P<0.01)。结论:不明原因复发性流产病人血液和绒毛蜕膜组织中的8-isoprostane、SOD、NF-κB和iNOS表达与正常妊娠妇女具有明显差异,因此,其可作为预测不明原因复发性流产发生的重要因子。Objective:To investigate the expression levels of 8-isoprostane,SOD,AOA,NF-kB and iNOS in patients with recurrent spontaneous abortion and their effects,and explore the pathogenesis of unexplained recurrent spontaneous abortion.Methods:From January 2018 to October 2019,119 maternal patients were selected,and divided into the case group(60 cases with unexplained recurrent abortion)and control group(59 cases with normal pregnancy delivery).The levels of 8-isoprostane,SOD,AOA and other oxidative stress indicators(NF-κB and iNOS)in the blood and villous decidua tissue were compared between two groups.Results:There was no statistical significance in the AOA level in blood between two groups(P>0.05),the levels of 8-isoprostane,NF-κB and iNOS in case group were significantly higher than those in control group(P<0.01),and the SOD level in case group was significantly lower than that in control group(P<0.01).The difference of the AOA level in chorion decidua tissue between two groups was not statistically significant(P>0.05),the levels of 8-isoprostane and NF-κin chorion decidua tissue in case group were higher than those in control group(P<0.01),and the level of SOD in case group was lower than that in control group(P<0.01).Conclusions:There are significant differences in the expression levels of 8-isoprostane,SOD,NF-κB and iNOS in blood and chorion decidua tissue between patients with unexplained recurrent abortion and normal pregnant women,and they can be used as an important factor to predict the occurrence of unexplained recurrent spontaneous abortion.

关 键 词：复发性流产 绒毛蜕膜组织 8-异构前列腺素 超氧化物歧化酶 血浆总抗氧化能力 转录因子-ΚB 诱导型一氧化氮合酶 

分 类 号：R714.21[医药卫生—妇产科学]