柚皮苷对破骨细胞分化和极化影响的初步探究  

Effects of naringin on differentiation and polarization of osteoclasts

作　　者：刘朝晖 马剑雄[2] 田爱现 孙晓雷[2] 张杨[2] 郭悦[2] 马信龙[1,2] LIU Zhao-hui;MA Jian-xiong;TIAN Ai-xian;SUN Xiao-lei;ZHANG Yang;GUO Yue;MA Xin-long(Department of Orthopedics,General Hospital of Tianjin Medical University,Tianjin 300052,China;Institute of Orthopedics,Tianjin Hospital)

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津市天津医院骨科研究所

出　　处：《天津医药》2020年第12期1141-1145,共5页Tianjin Medical Journal

基　　金：国家自然科学基金面上项目(81871777,11772226)。

摘　　要：目的探究柚皮苷对破骨细胞分化和极化功能的影响。方法体外培养RAW264.7细胞,设置对照组(培养基不含柚皮苷)和实验组(培养基中柚皮苷剂量分别为2、20、200μg/L)连续培养7 d,通过抗酒石酸酸性磷酸酶(TRAP)染色对比组间诱导分化结果,通过实时荧光定量逆转录聚合酶链反应(qPCR)对比组间组织蛋白酶K(CK)、基质金属蛋白酶-9(MMP-9)、整合素β3(integrinβ3)和非受体酪氨酸激酶(c-src)mRNA表达水平,通过蛋白免疫印迹法对比组间CK、MMP-9、integrinβ3、磷酸化产物p-src蛋白表达水平。结果与对照组相比,各实验组TRAP染色阳性细胞数显著降低(P<0.05);与对照组相比,20μg/L与200μg/L组CK、MMP-9、integrinβ3及c-src m RNA表达水平显著降低(P<0.05);与对照组相比,20μg/L与200μg/L组CK、MMP-9、integrinβ3及p-src蛋白表达水平显著降低(P<0.05)。结论柚皮苷对破骨细胞的分化和极化有一定的抑制作用,其作用机制可能与下调integrinβ3、c-src及p-src表达有关。Objective To investigate the effects of naringin on the differentiation and polarization of osteoclast.Methods RAW264.7 cells were induced using culture media containing different concentrations of naringin(0μg/L,2μg/L,20μg/L,200μg/L).The results of induction and expression levels of CK,MMP-9,integrinβ3,c-src and p-src were compared between groups.Results Compared with the control group,TRAP staining positive cell number was significantly reduced in the experimental group(P<0.05).Compared with the control group,the mRNA expression levels of CK,MMP-9,integrinβ3 and c-src were significantly reduced in 20μg/L and 200μg/L groups(P<0.05),and the protein expression levels of CK,MMP-9,integrinβ3 and p-src were also significantly reduced(P<0.05).Conclusion Naringin might inhibit the differentiation and polarization of osteoclast,and the effects might be related to the down regulation of integrinβ3,c-src and p-src expressions.

关 键 词：破骨细胞 骨质疏松 细胞分化 基因 SRC 整合素Β3 组织蛋白酶K 基质金属蛋白酶9 柚皮苷 

分 类 号：R285.5[医药卫生—中药学]