响应面法优化超声波辅助提取橡胶籽油及其脂肪酸成分分析  

Optimization of Ultrasonic-assisted Extraction of Rubber Seed Oil by Response Surface Methodology and Its Fatty Acid Composition Analysis

作　　者：杨晶晶 刘云 胡祥 朱义鑫 王云祥 阚欢 YANG Jing-jing;LIU Yun;HU Xiang;ZHU Yi-xin;WANG Yun-xiang;KAN Huan(College of Life Science,Southwest Forestry University,Kunming 650224,Yunnan,China;Huakun Biotechnology Co.,Ltd.,Xishuangbanna 666100,Yunnan,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]西双版纳华坤生物科技有限责任公司,云南西双版纳666100

出　　处：《食品研究与开发》2020年第21期137-144,共8页Food Research and Development

基　　金：云南森林培育协同创新中心(501355)。

摘　　要：以橡胶籽为原材料,通过超声波辅助溶剂法提取橡胶籽油。以橡胶籽油提取率为考察指标,在单因素试验的基础上,选取料液比、超声功率、超声时间、超声温度进行响应面优化试验;采用气相色谱法测定橡胶籽油脂肪酸组成。结果表明:石油醚提取效果最好,料液比1∶12.4(g/mL)、超声功率306 W、超声时间47 min、超声温度45.7℃,在此条件下橡胶籽油提取率可达42.80%。橡胶籽油主要是由棕榈酸(C16∶0)、硬脂酸(C18∶0)、油酸(C18∶1)、亚油酸(C18∶2)和α-亚麻酸(C18∶3)组成,不饱和脂肪酸含量达到79%以上。With rubber seed as material,rubber seed oil was extracted by ultrasonic-assisted solvent extraction method.On the basis of single factor test,material-liquid ratio,ultrasonic power,ultrasonic time and ultrasonic temperature were optimized by response surface method(RSM),rubber seed oil extraction rate as evaluation index;The fatty acids composition was determined by gas chromatography(GC).The research showed that petroleum ether was the best,the premium parameters for rubber seed extraction were the material-liquid ratio 1∶12.4(g/mL),ultrasonic power was 306 W,ultrasonic time for 47 min,ultrasonic temperature was 45.7℃.Under this circumstance,the rubber seed oil extraction rate of polyphenols could reach 42.80%.Results showed that major fatty acids were palmitic acid(C16∶0),stearic acid(C18∶0),oleic acid(C18∶1),linoleic acid(C18∶2)andα-linolenic acid(C18∶3)and the content of unsaturated fatty acids was more than 79%.

关 键 词：橡胶籽 橡胶籽油 超声波辅助提取 响应面 脂肪酸 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]