SDE葫芦巴叶挥发性提取物GC-MS分析与抗氧化活性研究  

GC-MS Analysis and the Antioxidant Activity of Volatile Components in Fenugreek Leaves by Simultaneous Distillation Extration

作　　者：李建芳 周枫 张阳阳 陈利军 刘坤峰 LI Jian-fang;ZHOU Feng;ZHANG Yang-yang;CHEN Li-jun;LIU Kun-feng(College of Food Science,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;Characteristic Food Resources Comprehensive Utilization Engineering Research Center of the Southern Region of Henan Province,Xinyang 464000,Henan,China;College of Agriculture,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;College of Food Science,Jiangnan University,Wuxi 214122,Jiangsu,China)

机构地区：[1]信阳农林学院食品学院,河南信阳464000 [2]河南省豫南特色食物资源综合利用工程技术研究中心,河南信阳464000 [3]信阳农林学院农学院,河南信阳464000 [4]江南大学食品学院,江苏无锡214122

出　　处：《食品研究与开发》2020年第21期154-160,共7页Food Research and Development

基　　金：国家自然科学基金(31401798);河南省高新技术领域科技攻关项目(172102210452);校级科技创新团队建设项目(CXTD-201802)。

摘　　要：以葫芦巴叶为研究对象,采用同时蒸馏萃取法,配合气相色谱-质谱技术(gas chromatography-mass spectrometer,GC-MS)系统分析葫芦巴叶中挥发性成分并研究其抗氧化活性。结果显示,同时蒸馏萃取法所得葫芦巴叶挥发性提取物为液态,呈黄色,得率为0.37%,具有浓郁的芳香气味且略带刺激性;GC-MS共检出23种化合物,包括腈类、萜烯类、酚类、醇类、醛类、醚类等。其中,含量最多的为苄基腈(42.914%),其次为(+)-4-蒈烯(11.749%)、桉叶醇(8.774%)、麝香草酚(5.321%)、2-甲氧基-4-乙烯基苯酚(4.582%)和香豆素(3.773%)等;对提取得到的挥发性物质进行抗氧化活性测定,结果显示,其总还原力、DPPH自由基清除能力、超氧阴离子自由基(O2-·)清除能力和ABTS+自由基(ABTS+·)清除能力与质量浓度均呈量效关系,但总体上,其抗氧化能力较阳性对照VC小。Fenugreek leaves were used as the study object,by simultaneous distillation extraction(SDE),and combined with gas chromatography-mass spectrometry(GC-MS),analyzed systematically the volatile components in fenugreek leaves.The experimental results showed that the volatile components of fenugreek leaves obtained by SDE showed faint yellow,liquid,at a rate of 0.37%,23 compounds were detected,including nitriles,terpenes,phenols,alcohols,aldehydes,ethers,et al.benzyl nitrile(42.914%),(+)-4-carene(11.749%),eucalyptol(8.774%),3-methyl-4-isopropylphenol(5.321%),2-methoxy-4-vinylphenol(4.582%)and coumarin(3.773%),et al.The antioxidant activity of the extracted volatile substances was determined.The experimental results show that its total reducing power,its the clearance of DPPH,its the cleanance of super oxide anion free radical scavenging(O2-·)and its the cleavance of ABTS free radical scavenging,and the mass concentration are dose-effect relationship,overall,to compare with VC,its antioxidant activity's lower.

关 键 词：葫芦巴叶 同时蒸馏萃取法(SDE) 挥发性提取物 气相色谱-质谱联用(GC-MS) 抗氧化活性 

分 类 号：O657.63[理学—分析化学] R284[理学—化学]