苹果属植物DNA去甲基化基因MdIDM1参与低温促进花色素苷的积累  

DNA demethylated gene MdIDM1 promoted anthocyanin accumulation in Malus plants under low-temperature stress

作　　者：孙玉莹 于璐佳 张杰 SUN Yuying;YU Lujia;ZHANG Jie(College of Plant Science and Technology/Beijing Key Laboratory of New Technology in Agricultural Application,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院植物科学技术学院/农业应用新技术北京市重点实验室,北京102206

出　　处：《北京农学院学报》2021年第1期1-7,共7页Journal of Beijing University of Agriculture

基　　金：长城学者项目(5045243001)。

摘　　要：【目的】为探明DNA去甲基化基因与苹果属植物低温诱导花色素苷积累的关系。【方法】以苹果‘嘎啦’和观赏海棠‘王族’与观赏海棠‘火焰’组培苗叶片为试材,16℃低温连续处理7 d,通过RNA提取和反转录cDNA,克隆得到苹果属植物DNA去甲基化关键基因MdIDM1,通过荧光定量PCR和高效液相色谱分别检测叶片中花色素苷合成基因和花色素苷含量,对去甲基化基因MdIDM1表达量进行相关性分析。【结果】低温胁迫促进苹果属植物着色,同时随着低温处理时间的增加MdIDM1的表达逐渐升高,且与花色素苷生物合成基因表达和花色素苷积累呈正相关。【结论】低温条件下,DNA去甲基化基因MdIDM1可能参与苹果属植物花色素苷积累。【Objective】DNA demethylation gene was cloned to investigate the role of DNA demethylation in anthocyanin accumulation under low temperature in Malus plants.【Methods】The leaves of Malus domestica cv.‘Gala’,Malus cv.‘Royalty’and Malus cv.‘Flame’were used as experimental materials.The leaves of apple plants were exposured to 16℃low temperature for 7 days,the anthocyanin synthesis gene and anthocyanin content in the leaves of apple‘Gala’and crabapple‘Royalty’and‘Flame’cultured seedlings were determined by quantitative PCR and high performance liquid chromatography.Through RNA extraction and reverse transcription of cDNA,the key DNA demethylated gene MdIDM1(Increased DNA Methylation 1)was cloned,and the correlation analysis between the expression of demethylated gene MdIDM1 and the accumulation of anthocyanin and the transcription of anthocyanin related genes were conducted.【Results】Low temperature promoted anthocyanin accumulation of Malus plants.Meanwhile,the expression of MdIDM1 gene increased with the low temperature treatment time,and it was positively correlated with the expression of anthocyanin biosynthesis gene and accumulation of anthocyanin.【Conclusion】DNA demethylated gene MdIDM1 may be play an important role in low temperature mediated anthocyanin accumulation in the Malus plants.

关 键 词：苹果 低温胁迫 DNA去甲基化 花色素苷代谢途径 

分 类 号：S661.1[农业科学—果树学]