土壤多组分PAHs激光诱导二维荧光光谱定量方法研究  

Study on quantitative methods of laser-induced two-dimensional fluorescence spectroscopy of multicomponent PAHs in soils

作　　者：黄尧 赵南京[1,3] 孟德硕 左兆陆 程钊 陈宇男 陈晓伟 谷艳红 HUANG Yao;ZHAO Nan-jing;MENG De-shuo;ZUO Zhao-lu;CHENG Zhao;CHEN Yu-nan;CHEN Xiao-wei;GU Yan-hong(Key Laboratory of Environmental Optics&Technology,Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Hefei 230031,China;University of Science and Technology of China,Hefei 230026,China;Key Laboratory of Optical Monitoring Technology for Environment of Anhui Province,Hefei 230031,China;Hefei University,Institute of Advanced Manufacturing Engineering,Hefei 230601,China)

机构地区：[1]中国科学院安徽光学精密机械研究所环境光学与技术重点实验室,合肥230031 [2]中国科学技术大学,合肥230026 [3]安徽省环境光学监测技术重点实验室,合肥230031 [4]合肥学院先进制造工程学院,合肥230601

出　　处：《中国光学》2020年第6期1401-1410,共10页Chinese Optics

基　　金：Supported by National Natural Science Foundation of China(No.61705238);National Key Research and DevelopmentProgram of China(No.2016YFD800902-2);Anhui Science and Technology Major Projects(No.904038316006);Natural Science Foundation of Anhui Province(No.2008085QF316)。

摘　　要：激光诱导荧光技术具有实时、快速的优势,并且无需对样品做预处理,是土壤多环芳烃定量分析检测的一种重要分析手段。然而土壤中多环芳烃种类繁多,激光诱导荧光光谱重叠严重,在无法进行化学分离的情况下实现土壤中多环芳烃的精确定量是难点之一。本文采用266 nm可移动激光诱导荧光系统获取了农田土壤多环芳烃的荧光光谱,研究了基于单变量线性回归、加权非负最小二乘多元线性回归和支持向量回归的多组分多环芳烃定量分析方法。结果表明:采用单变量线性回归,蒽和菲的相关系数均小于0.90,平均相对误差均大于20%;与单变量线性回归相比,加权非负最小二乘多元线性回归提高了两组分多环芳烃污染土壤中蒽和菲的预测精度,但在多组分多环芳烃污染土壤中的平均相对误差仍在20%以上。最后,采用GWO-DE优化的支持向量机回归模型分析了多组分多环芳烃污染土壤中的蒽和菲,蒽的平均相对误差由多元线性回归的23.1%下降至5.02%,菲的平均相对误差从20.8%下降到4.83%。该研究为提高土壤多组分多环芳烃激光诱导荧光定量分析的准确性提供了方法支撑。Laser-Induced Fluorescence(LIF)has become a powerful technology for quantitative analysis of Polycyclic Aromatic Hydrocarbons(PAHs)in soils due to its fast detection speed and low operational cost,without sample preparation.However,there are many types of PAHs in soils,and their similar structures lead to overlapped issues in their laser-induced fluorescence spectra.It is challenging to quantify a single PAH in complicated soil without the chemical separation.In this paper,fluorescence spectra of PAHs in agricultural soil are obtained by a 266 nm mobile LIF system,and quantification methods are investigated for PAHs,based on univariate linear regression,weighted non-negative least squares Multivariate Linear Regression(MRL)and Support Vector Regression(SVR).The results show that the correlation coefficients of anthracene and phenanthrene are both less than 0.90,and the average relative errors are both more than 20%by using univariate linear regression.Compared with univariate linear regression,MLR improves the prediction accuracy of anthracene and phenanthrene in the soil contaminated with bi-component PAHs.However,the average relative errors are still over 20%in the soil contaminated with multicomponent PAHs.Finally,a SVR model optimized by grey wolf optimization combined with differential evolution(GWO-DE)is applied for concentration measurement of anthracene and phenanthrene in agricultural soil contaminated with multicomponent PAHs.The average relative error of anthracene decreases from 23.1%(MLR)to 5.02%,while in the case of phenanthrene decreases from 20.8%(MLR)to 4.83%.This study provides an efficient method to improve the accuracy of LIF in quantifying multicomponent PAHs in soils.

关 键 词：多环芳烃 激光诱导荧光光谱 定量分析 

分 类 号：O657.319[理学—分析化学]