补阳还五汤辅助鼠源MSCs移植治疗SD大鼠脊髓损伤的研究  

Study on the treatment of spinal cord injury in SD rats by Buyang Huanwu decoction-assisted rat-derived MSCs transplantation

作　　者：王璐瑶 朱佳文 张文远 王天枢[1] WANG Luyao;ZHU Jiawen;ZHANG Wenyuan;WANG Tianshu(Department of Brain Surgery,the First Hospital affiliated to Jiamusi University,Heilongjiang Jiamusi 154002,China)

机构地区：[1]佳木斯大学附属第一医院脑外科,黑龙江佳木斯154002

出　　处：《上海医药》2020年第23期39-42,共4页Shanghai Medical & Pharmaceutical Journal

基　　金：黑龙江省卫生计生委科研课题(201810222058)。

摘　　要：目的:探讨大鼠脊髓损伤(spinal cord injury,SCI)后神经干细胞(neural stem cells,MSCs)移植与补阳还五汤(Buyang Huanwu decoction,BYHWD)对大鼠脊髓全横断损伤的修复。方法:对脊髓损伤的大鼠分别进行鼠源MSCs移植或补阳还五汤治疗及两者联合治疗,观察治疗后大鼠行为学变化;检测各组细胞wnt3a与β-actin比值。结果:t检验分析表明,MSCs+BYHWD组大鼠的BBB评分值高于脊髓损伤组[(17.77±0.97)vs(15.33±1.02),P<0.05];Western-Blot结果表明,与对照组相比,MSCs组、BYHWD组及MSCs+BYHWD组的wnt3a表达上调,且MSCs+BYHWD组远高于MSCs组和BYHWD组。结论:补阳还五汤辅助MSCs移植治疗SD大鼠脊髓损伤有一定改善效果,且改善效果远高于MSCs或BYHWD单药治疗。Objective:To investigate the effect of neural stem cell(MSCs)transplantation and Buyang Huanwu decoction(BYHWD)on spinal cord injury(SCI)in rats.Methods:The rats with spinal cord injury were treated with rat-derived MSCs transplantation or Buyang Huanwu decoction and the combination of the two therapies.The behavioral changes of the rats were observed and the ratios of wnt3a toβ-actin in each group of cells were detected.Results:The student’s t tests showed that the BBB score of the rats was higher in the MSCs+BYHWD group than the SCI group[(17.77±0.97)vs(15.33±1.02),P<0.05].Western-Blotting results showed that the expression of wnt3a in the MSCs group,BYHWD group and MSCs+BYHWD group was up-regulated as compared with the control group and furthermore its expression was much higher in the MSCs+BYHWD group than the MSCs group and BYHWD group.Conclusion:Buyang Huanwu decoction-assisted MSCs transplantation for the treatment of SCI in rats has certain improvement effect and its effect is much higher compared with MSCs and BYHWD monotherapy.

关 键 词：MSC 脊髓损伤 SD 大鼠 补阳还五汤 

分 类 号：R289.5[医药卫生—方剂学] Q813.3[医药卫生—中药学]