三聚氰胺与牛血清白蛋白相互作用的光谱学研究  

Spectroscopic studies of interaction between melamine and bovine serum albumin

作　　者：杨美玲[1] 崔东亚[1] 牛润华 YANG Mei-ling;CUI Dong-ya;NIU Run-hua(Department of Applied Chemistry,Yuncheng University,Yuncheng 044000,Shanxi,China)

机构地区：[1]运城学院应用化学系,山西运城044000

出　　处：《湖北农业科学》2020年第21期163-167,共5页Hubei Agricultural Sciences

基　　金：运城学院院级支撑项目(YQ-2015010)。

摘　　要：在模拟生理条件下,采用紫外吸收光谱法、荧光光谱法和圆二色谱法研究了三聚氰胺与牛血清白蛋白(Bovine serum albumin,简称BSA)之间的相互作用。紫外吸收光谱法和荧光光谱法的研究结果表明,三聚氰胺与牛血清白蛋白相互作用可在基态时形成一种新的复合物从而导致牛血清白蛋白的荧光猝灭,其猝灭方式为静态猝灭。经过计算可得出,在28、32、37℃其猝灭常数分别为1.618×10^(12)、1.614×10^(12)、1.620×10^(12)L/(mol·s);其结合常数分别为2.64×10~3、7.59×10~3、9.80×10~3L/mol,结合位点数约为1,结合距离为3.7 nm,说明三聚氰胺不仅可以和BSA结合形成新的复合物,而且BSA上的1个位点只结合1个三聚氰胺药物分子,不同结合位点之间的结合距离为3.7 nm。而圆二色谱法的研究结果显示,三聚氰胺对牛血清白蛋白的二级结构具有较大的影响。The interaction between melamine and bovine serum albumin(BSA)was investigated by using fluorescence spectroscopy,UV absorption spectroscopy and circular dichroism spectroscopy with the simulated physiological condition.The experiment results of UV absorption spectroscopy and fluorescence spectroscopy exhibited that melamine and BSA could form a kind of new compound that could be proved through the fluorescence quenching of the compound of BSA and melamine.It was the static fluorescence quenching.According to the calculation,at 28,32 and 37℃,the quenching constants were 1.618×1012,1.614×1012,1.620×1012 L(/mol·s),re⁃spectively;The binding constants were 2.64×103,7.59×103 and 9.80×103 L/mol,respectively;And the number of binding sites was 1,approximately;And the binding distance was 3.7 nm.All these indicated that a new chemical compound was produced in the mixture of BAS and melamine,and a special binding sit of BAS could only bind a one melamine molecule,and the binding distance between different sites was 3.7 nm.The results of circular dichroism displayed that melamine had a great effect on the secondary structure of BSA.

关 键 词：牛血清白蛋白 三聚氰胺 荧光光谱法 紫外吸收光谱法 圆二色谱法 

分 类 号：O657.3[理学—分析化学]