基于染色体片段代换系的水稻穗部性状QTL定位  

QTL Mapping of Panicle Traits Based on Rice Chromosome Substitution Lines

作　　者：杨旭东[1] 赵芳明[2] 罗洪发[1] 赵福胜[1] 潘清洁 张治海 查仁明[1] YANG Xudong;ZHAO Fangming;LUO Hongfa;ZHAO Fusheng;PAN Qingjie;ZHANG Zhihai;ZHA Renming(College of Agriculture,Guizhou University,Guiyang,Guizhou 550025,Chna;Rice Research Institute,Southwest University/Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture,Chongqing 400716,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]西南大学水稻研究所/农业部生物技术与作物品质改良重点开放实验室,重庆400716

出　　处：《山地农业生物学报》2020年第5期78-81,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技厅项目(黔教合KY字[2015]333);中央高校基本科研业务费专项“创新团队”项目(XDJK2017A004)。

摘　　要：水稻穗部性状与产量直接相关,筛选控制水稻穗部性状的基因对于水稻育种具有重要意义。本研究以日本晴为母本,染色体片段代换系Z1347为父本构建F_2群体,用SSR标记对穗长、二次枝梗数等穗部性状进行了QTL定位。结果表明:Z1347的穗长和二次枝梗数均显著高于日本晴,但两者的一次枝梗数无差异;SSR标记检测出5个QTL,其分布于水稻第1、6、7和11染色体上,其中与穗长相关的qPL1、qPL6、qPL7和qPL11等4个QTL,分别与RM5389、RM2126、RM6063和RM457连锁,与二次枝梗数相关的QTL (qNSB7)与RM6063连锁。5个QTL中,qPL11和qNSB7可能为新的位点,可在后续试验中加强其遗传效应的研究,为进一步的分子辅助育种提供参考。Rice panicle traits are directly related to yield.Screening out the genes that control rice panicle traits are important for analyzing the molecular mechanisms and breeding of their cultivars.In this study,Nipponbare and the chromosome fragment substitution line Z1347 were used as the female parent and male parent to construct the F 2 population.QTL mapping of panicle traits of panicle length,number of primary branches and number of secondary branches were performed using SSR markers.The results showed that the number of panicle length and secondary branches of Z1347 were significantly higher than that of Nipponbare.However,there was no difference in the number of primary branches between two cultivars.SSR markers detected 5 QTLs which were distributed on chromosomes 1,6,7,and 11.Among them,4QTLs(qPL1,qPL6,qPL7,and qPL11)related to the panicle length were linked to RM5389,RM2126,RM6063 and RM457,respectively.The QTL of qNSB7 related to the secondary branch number was linked to RM6063.Among the 5 QTLs,qPL11 and qNSB7 might be new location,which can be used to strengthen the research of their genetic effects in subsequent experiments and provide references for further molecular-assisted design breeding.

关 键 词：QTL 染色体片段代换系 穗部性状 水稻 

分 类 号：S336[农业科学—作物遗传育种]