黄麻应用核心种质的DNA分子身份证构建  

Establishment of DNA molecular fingerprint of applied core germplasm in jute(Corchorus spp.)

作　　者：郭艳春 张力岚 陈思远[1,2,3] 祁建民 方平平 陶爱芬[1,2] 张列梅 张立武[1,2,3] GUO Yan-Chun;ZHANG Li-Lan;CHEN Si-Yuan;QI Jian-Min;FANG Ping-Ping;TAO Ai-Fen;ZHANG Lie-Mei;ZHANG Li-Wu(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops/Fujian Key Laboratory for Crop Breeding by Design,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Public Platform of Fujian for Germplasm Resources of Bast Fiber Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Center for Genomics and Biotechnology,Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学农学院作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室,福建福州350002 [2]福建农林大学农业农村部东南黄红麻实验观测站/福建省麻类种质资源共享平台/福建省南方经济作物遗传育种与多用途开发国际科技合作基地,福建福州350002 [3]福建农林大学海峡联合研究院基因组与生物技术中心,福建福州350002

出　　处：《作物学报》2021年第1期80-93,共14页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31771369);国家现代农业产业技术体系建设专项(CARS-19-E06)资助。

摘　　要：构建并研究黄麻应用核心种质是促进黄麻遗传育种和挖掘优异基因的必要途径。在300份黄麻种质资源的农艺性状观察统计基础上,构建了黄麻应用核心种质,包含61份品种(系),可划分为高产、优质、抗病等16种应用类型。为准确鉴定这61份应用核心种质,以46对核心引物为基础,筛选出12对荧光核心引物,采用荧光标记毛细管电泳分析这12对引物的多态性,共检测出140个多态性位点。将毛细管电泳得到的分子量数据以数字+英文字母方式编码,选取了12对荧光核心引物的组合,构建出该应用核心种质的字符串DNA分子身份证,进而构建了相应的条形码和二维码DNA分子身份证,可迅速被电子设备识别。这些结果可促进黄麻种质资源的高效利用及快速分子鉴定。It is important to construct and study the applied core collection in order to promote jute genetic breeding and explore excellent genes.In this study,the applied core collection in jute,including 61 accessions divided into 16 application-oriented features,such as high yield,high quality,disease resistance and so on,was established based on the field performance from 300 jute germplasm.12 fluorescent core primers were screened from 46 pairs of core primers.Fluorescence labeled capillary electrophoresis was used to analyze the polymorphism of these 12 pairs of primers,and a total of 140 polymorphism sites were detected.The precise DNA molecular ID cards were constructed by the combination of the 12 core primer pairs from the data coded in the form of numbers and English letters.Bar code and quick response code DNA molecular were established and can be quickly scanned and recognized by electric gadget.These results could be beneficial to increase the application efficiency and rapid molecular identification in jute germplasm resources.

关 键 词：黄麻 应用核心种质 SSR 荧光分子标记 DNA分子身份证 

分 类 号：S563.4[农业科学—作物学]