基于90K芯片的小麦穗长和旗叶长QTL分析  

QTL Analysis of Wheat Spike Length and Flag Leaf Length Based on 90K SNP Assay

作　　者：姚俭昕 张传量 宋晓朋 许小宛 邢永锋 吕栋云 宋鹏博 杨孟于 孙道杰[1] YAO Jianxin;ZHANG Chuanliang;SONG Xiaopeng;XU Xiaowan;XING Yongfeng;LU Dongyun;SONG Pengbo;YANG Mengyu;SUN Daojie(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;Zhoukou Academy of Agricultural Science,Zhoukou,Henan 466000,China;Zhumadian Academy of Agricultural Science,Zhumadian,Henan 463000,China)

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]周口市农业科学院,河南周口466000 [3]驻马店市农业科学院,河南驻马店463000

出　　处：《麦类作物学报》2020年第11期1283-1289,共7页Journal of Triticeae Crops

基　　金：国家重点研发计划项目(2016YFD0101802);陕西省重点研发计划项目(2018DXM-NY-010)。

摘　　要：为进一步挖掘控制小麦穗长和旗叶长的QTL,以小偃81和西农1376构建的包含120个株系的F 9∶10 RIL群体为材料,于2016年10月至2017年6月和2017年10月至2018年6月分别在陕西杨凌和河南南阳(用2017YL、2017NY、2018YL和2018NY表示)进行试验,对4个环境下的穗长和旗叶长进行表型鉴定,并利用90K芯片构建的高密度遗传连锁图谱进行QTL定位。结果表明,所构建的遗传图谱覆盖了小麦21条染色体,图谱全长3172.49 cM,平均图距0.57 cM。采用完备复合区间模型对4种环境下的表型值及BLUP育种值分别进行QTL定位,共检测到3个控制穗长的QTL,分布在2B、2D和5D染色体上;检测到2个控制旗叶长的QTL,均分布在5A染色体上,其中控制穗长的Qsl.nwsuaf-2D、Qsl.nwsuaf-5D和控制旗叶长的Qfll.nwsuaf-5A.1能够在多环境下稳定表达,为主效QTL,表型变异解释率分别为18.34%~22.51%、9.57%~14.94%和9.48%~16.36%。该结果可为小麦MAS育种、NIL构建、候选区域筛选及图位克隆等提供参考依据。In order to further excavate the QTLs of wheat ear length and flag leaf length,the F 9∶10 RIL population consisting of 120 lines constructed by Xiaoyan 81 and Xinong 1376 was used as material and were planted in Yangling,Shaanxi and Nanyang,Henan from October 2016 to June 2017 and from October 2017 to June 2018(represented as 2017YL,2017NY,2018YL and 2018NY,respectively).The phenotyping of spike length and flag leaf length under the four environments was carried out,the high-density genetic linkage map constructed by 90K SNP assay was used for QTL mapping.The results showed that the generated genetic map covers 21 wheat chromosomes;the linkage group is 3172.49 cM,and the average map distance is 0.57 cM.A complete compound interval model was used for QTL mapping analysis with the phenotypic values and BLUP breeding values under the four environments.3 QTLs controlling spike length were detected on 2B,2D and 5D chromosomes,and 2 QTLs controlling flag leaf length were detechted on 5A chromosome.Qsl.nwsuaf-2D,Qsl.nwsuaf-5D(controlling spike length)and Qfll.nwsuaf-5A.1(controlling flag leaf length)detechted multiple environments were major QTLs,explaining the phenotypic variation ranging from 18.34%to 22.51%,9.57%to 14.94%and 9.48%to 16.36%,respectively.The results provide reference for wheat MAS breeding,NIL construction,candidate region screening and gene cloning.

关 键 词：小麦 穗长 旗叶长 QTL 

分 类 号：S512.1[农业科学—作物学] S330