氮浓度对新型生物电化学-颗粒污泥反应器运行的影响  

Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor

作　　者：邓秋金 宿程远 陆欣雅[2] 关鑫 覃容华 邓钰莲 高澍 黄尊 DENG Qiujin;SU Chengyuan;LU Xinya;GUAN Xin;QIN Ronghua;DENG Yulian;GAO Shu;HUANG Zun(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guilin 541004,Guangxi,China;School of Environment and Resources,Guangxi Normal University,Guilin 541004,Guangxi,China)

机构地区：[1]珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004

出　　处：《化工学报》2020年第12期5802-5812,共11页CIESC Journal

基　　金：国家自然科学基金项目(51768009);广西研究生创新项目学位与研究生教育改革课题(JGY2019035);广西师范大学青年基金项目(2016QN005)。

摘　　要：探讨了新型生物电化学-颗粒污泥反应器在不同进水氮浓度下的脱氮效能与产电性能,并从颗粒污泥的关键酶活性、胞外聚合物组分以及微生物群落分布等角度系统研究了其影响机制。结果表明,COD、NO3^--N、NO2--N和溶解性甲烷在第Ⅰ、Ⅱ、Ⅲ、Ⅳ阶段(进水NO3^--N和NO2^--N浓度分别为60 mg·L-1和20 mg·L^-1、100 mg·L^-1和40 mg·L^-1、140 mg·L^-1和60 mg·L^-1、180 mg·L^-1和80 mg·L^-1)均得以有效去除,其中COD去除率在第Ⅳ阶段效果最佳,去除率达96%以上,NO3^--N出水浓度在第Ⅱ阶段更为稳定,其去除率达99%以上,NO2--N去除率在各阶段均达99%以上;该反应器最大的功率密度与输出电压值为第Ⅳ阶段的4号格室,分别为471.2 mV·m^-3和608.1 mV。污泥疏松型胞外聚合物(LB-EPS)中多糖与蛋白含量最高为第Ⅱ阶段的5号格室,分别为13.7 mg·g^-1和14.7 mg·g^-1;1号格室污泥中辅酶F420活性最低,进水氮浓度的增大提高了污泥中蛋白酶活性。由第Ⅰ阶段至第Ⅳ阶段,该反应器中变形菌门(Protebaoteria)相对丰度减少,而绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和浮霉菌门(Planctomycetes)相对丰度增加;具有脱氮作用的陶厄氏菌属(Thauera)在1号格室减少了8.64%,但该反应器脱氮效果未受到影响;甲烷丝状菌属(Methanothrix)在4号格室相对丰度增至12.3%,表明产甲烷菌可在该反应器中与其他菌群联营共存。The performance of nitrogen removal and electricity generation of a novel bio-electrochemical-granular sludge reactor at different influent nitrogen concentration was investigated.The impact mechanism of granular sludge,key enzyme activity,extracellular polymer composition and microbial community distribution were systematically studied.The results showed that COD,NO3^--N,NO2^--N and dissolved methane were efficiently removed in stagesⅠ,Ⅱ,Ⅲ and Ⅳ(Influent NO3^--N and NO2^--N concentrations were 60 and 20 mg·L^-1,100 and 40 mg·L-1,140 and 60 mg·L^-1,180 and 80 mg·L^-1,respectively).The removal efficiency of COD was the highest in stageⅣ,and it was above 96%.The effluent concentration of NO3--N was the most stable at stageⅡ,and the removal efficiency was over 99%.The NO2--N removal efficiency was above 99%in each stage.In stageⅣ,the maximum power density and output voltage was 471.2 mV·m-3 and 608.1 mV at the fourth compartment,respectively.The polysaccharide and protein content of LB-EPS was the highest in stage Ⅱ of the fifth compartment,13.7 mg·g^-1 and 14.7 mg·g^-1,respectively.Coenzyme F420 activity was the lowest in the first compartment.The protease activity of the sludge was increased due to the increase of influent nitrogen concentration.From stage Ⅰ to stage Ⅳ,the relative abundance of Protebaoteria was decreased,while the relative abundance of Chloroflexi,Firmicutes and Planctomycetes were increased.Although Thauera with denitrification effect was decreased by 8.64%in the first compartment,the nitrogen removal was still well in the reactor.The relative abundance of Methanothrix was increased to 12.3%in the fourth compartment,indicating that Methanothrix could co-exist with other bacteria in the reactor.

关 键 词：电化学 厌氧 氮浓度 废水 微生物群落 

分 类 号：X703.1[环境科学与工程—环境工程]