Metabolic profiling of DREB-overexpressing transgenic wheat seeds by liquid chromatographymass spectrometry  

作　　者：Fengjuan Niu Qiyan Jiang Xianjun Sun Zheng Hu Lixia Wang Hui Zhang 

机构地区：[1]Institute of Crop Sciences,National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2020年第6期1025-1036,共12页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31601302);National Transgenic Key Project from the Ministry of Agriculture of China(2016ZX08011-003);National Key Research and Development Program of China(2016YFD0100304);the Agricultural Science and Technology Program for Innovation Team(Evaluation on the Quality and Stress Tolerance of Crop Germplasm),CAAS。

摘　　要：DREBs are transcription factors that regulate abiotic stress tolerance in plants.Previously,we reported that wheat transgenic lines overexpressing Gm DREB1 showed increased tolerance to drought and salt stress.However,the molecular basis of increased tolerance is still poorly understood,and whether the overexpression of DREB will cause unexpected effects is also of concern.We performed seed metabolic profiling of the genetically modified(GM)wheat T349 and three non-GM cultivars with LC-MS to identify the metabolic basis of stress tolerance and to assess the unexpected effects of exogenous gene insertion.Although we did not note the appearance of novel metabolites or the disappearance of existing metabolites,overexpression of the transcription factor Gm DREB1 in T349 wheat influenced metabolite levels in seeds.Increased levels of stress tolerance-associated metabolites were found in the stress-sensitive non-transgenic acceptor counterpart J19,while metabolites associated with cell membrane structure and stability accumulated in T349.Among these metabolites in T349,most showed levels similar to those in the non-GM wheats.Overexpression of Gm DREB1 in T349 may cause a shift in its metabolic profile leading to down-regulation of several energy-consuming processes to favor increased yield under stress conditions,which is a reasonable expectation of breeders while creating the GM wheat and Gm DREB1 overexpression did not cause unexpected effects in T349 seeds.These results may be helpful for GM crop research and risk assessment.

关 键 词：Transgenic wheat Metabolomics LC-MS DREB Drought tolerance 

分 类 号：S512.1[农业科学—作物学]