Identification of a novel planthopper resistance gene from wild rice(Oryza rufipogon Griff.)  

作　　者：Meng Yang Jiebin Lin Ling Cheng Hailian Zhou Shu Chen Fang Liu Rongbai Li Yongfu Qiu 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Agricultural College,Guangxi University,Nanning 530005,Guangxi,China [2]College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China

出　　处：《The Crop Journal》2020年第6期1057-1070,共14页作物学报（英文版）

基　　金：supported by National Key Research and Development Program of China(2016YFD0100600);the National Program on Research and Development of Transgenic Plants(2014ZX0800911B);the National Natural Science Foundation of China(31160276 and 31560423);the Guangxi Innovation-Driven Development Special Funding Project(Guike-AA17204070);the State Key Laboratory of Hybrid Rice(KF201905)。

摘　　要：Rice planthoppers,including brown planthopper(BPH)and white-backed planthopper(WBPH),are the most destructive pests in Asian rice cultivation regions.Planthopper resistance genes that have been mapped and characterized advance our understanding of underlying resistance mechanisms and facilitate the breeding of resistant varieties,thereby contributing to an efficient pest management strategy.In this study,a novel resistance gene Bph38 derived from the wild rice species Oryza rufipogon Griff.was found to confer high resistance to BPH and WBPH.Conventional mapping was performed to identify regions associated with BPH and WBPH resistance,and two mapping efforts led to the same region on chromosome 4 flanked by markers RM16563 and RM16763.Bulked-segregant analysis and next-generation sequencing were performed using the same population to detect the resistance gene.Conventional mapping narrowed the region to a 12.3-Mb segment,and fine mapping using BC1 F2 recombinants identified a 79-kb segment flanked by markers YM112 and YM190.Near-isogenic lines(NILs)carrying Bph38 in the 9311(indica)and BR54(japonica)genetic backgrounds were developed by crossing and backcrossing with marker-assisted selection.The agronomic traits and BPH and WBPH resistance of the NILs were similar to those of the recurrent parents.Mandatory feeding and host-choice tests revealed that Bph38 showed both antibiotic and antixenotic effects in both insects,with stronger effects in indica-background lines.Further fine mapping and characterization of the major gene may result in map-based cloning of the gene and allow its application in breeding insectresistant rice varieties.

关 键 词：Oryza rufipogon Griff. Planthopper resistance Next-generation sequencing-based bulked-segregant analysis Gene mapping Resistance mechanism 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]