甘薯糖蛋白分离纯化及结构分析  

Study on Isolation Purification and Identification of Sweet Potato Glycoprotein

作　　者：孙昌平 王贵珍 SUN Changping;WANG Guizhen(Changchnu Food and Drug Administration,Automobile Economic and Technological Development Zone Supervision and Administration Institute,Changchun,Jilin 130000,China;College of Food Engineering,Jilin Engineering Normal University,Changchun,Jilin 130000,China)

机构地区：[1]长春市食品药品监督管理局汽车经济技术开发区监督管理所,吉林长春130000 [2]吉林工程技术师范学院食品工程学院,吉林长春130000

出　　处：《农产品加工》2020年第21期55-58,共4页Farm Products Processing

基　　金：吉林省教育厅“十三五”科学研究规划项目(JJKH2020185KJ);博士科研启动经费专项项目(BSKJ201922)。

摘　　要：根据甘薯糖蛋白易溶于水的性质,以水为浸提剂提取,经DEAE-22纤维素离子交换柱层析和Sephadex G-150葡聚糖凝胶柱层析得糖蛋白纯品。在此基础上,测定了蛋白质和多糖的含量,分析了氨基酸的组成、糖苷键类型,结果表明,甘薯糖蛋白中糖含量为75.1%,蛋白含量为23.9%,含有人体所必需的7种氨基酸,含有α-糖苷键。We extracted glycoprotein from sweet potato by using water as agent based on its water-soluble property,and harvested purified sample based on DEAE-22 cellulose ion exchange column chromatography and Sephadex G-150 glucan gel column chromatography.Furthermore,we detected the content of polysaccharide and protein,analyzed the composition of amino acid,the type of glycosidic bond and found that the content of polysaccharide and protein were 75.1%and 23.9%respectively.The glycoprotein contained seven amino acids that are essential for human body andα-glycosidic bond.

关 键 词：甘薯 糖蛋白 分离 纯化 结构分析 

分 类 号：TQ936[化学工程]