基于UPC^2-DAD对大黄药材中蒽醌类化合物的快速检测  

Retention Behavior and Rapid Detection of Anthraquinones in Rhubarb by Ultra Performance Convergence Chromatography

作　　者：李晓蓉[1] 蔡伟 张淼 周小平 黄铮[1] 徐美蓉[1] 李婷[1] 王波 LI Xiaorong;CAI Wei;ZHANG Miao;ZHOU Xiaoping;HUANG Zheng;XU Meirong;LI Ting;WANG Bo(Institute of Animal and Grass and Green Agriculture,Gansu Academy of Agricultural Sciences(Institute of Agricultural quality Standards and testing Technology,)Lanzhou,Gansu 730020,China;Gansu Agricultural Vocational and Technical College,Lanzhou,Gansu 730010,China;Lixian Spring Pharmaceutical Co.,Ltd.,Longnan,Gansu 742200,China;Lanzhou Customs Technology Center,Lanzhou,Gansu 730010,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所(农业质量标准与检测技术研究所),甘肃兰州730020 [2]甘肃省农业职业技术学院,甘肃兰州730010 [3]礼县春天药业有限责任公司,甘肃陇南742200 [4]兰州海关技术中心,甘肃兰州730010

出　　处：《农产品加工》2020年第21期72-78,共7页Farm Products Processing

基　　金：兰州海关科技计划项目(2017GK005,2017GK006)。

摘　　要：首次建立了超高效合相色谱法(Ultra Performance Convergence Chromatography,UPC2)快速检测大黄中5种蒽醌类化合物的方法。大黄样品用甲醇提取后,以CO2和甲醇为流动相和改性剂,梯度洗脱,色谱柱为HSS SB C18,柱温40℃,流速2.0 mL/min,进样量1.0μL,检测波长260 nm;整个分析过程仅需1.6 min。该方法的线性范围除大黄酸为1.0~100.0 mg/kg外,其余均为0.5~100.0 mg/kg,日内及日间精密度的RSD范围分别为1.8%~3.5%和0.9%~1.2%。在最优条件下,对10批不同产地的大黄进行检测。结果表明,UPC2方法具高效快速、操作简单、试验成本低等优点,可以满足大黄中5种蒽醌类化合物的快速检测。Based on Ultra performance convergence chromatography,sub-2μm particles were used as column.The retention behavior of anthraquinones represented by emodin and its related substances under different separation conditions was studied for the first time,and the rapid separation of five anthraquinones in rhubarb samples was realized.In the tested stationary phase,using carbon dioxide as the mobile phase,methanol as the cosolvent completed the rapid separation of the target in the rhubarb sample within 1.6 min,and the method was verified.And the method verification was carried out.The linear range of the method was in the range of 1.0~100.0 mg/kg,the others was 0.5~100.0 mg/kg,and the RSD ranges of the intra-day and the inter-day precision were between 1.8%~3.5%and 0.9%~1.2%,respectively.The results showed that the method of UPC^2 had the advantages of high efficiency,simple operation and low experimental cost,and can be used for the rapid detection of five anthraquinones in rhubarb.

关 键 词：超高效合相色谱 蒽醌类化合物 快速检测 大黄 

分 类 号：O657.99[理学—分析化学] R284.1[理学—化学]