平肥2号方对肥胖大鼠血清瘦素及下丘脑p-STAT3和SOCS3表达的影响研究  

Research on the Effect of Pingfei No.2 Recipe on Serum Leptin and Expression of STAT3 and SOCS3 in Hypothalamus of Obese Rats

作　　者：杜佳[1] 简小兰[2] 李克雄 刘霞 何军锋[1] DU Jia;JIAN Xiaolan;LI Kexiong;LIU Xia;HE Junfeng(Diagnostics of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Hunan Province,Changsha 410208,China;Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine,Hunan Province,Changsha 410006,China;Graduate School,Hunan University of Chinese Medicine,Hunan Province,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医诊断学科,湖南长沙410208 [2]湖南省中医药研究院附属医院,湖南长沙410006 [3]湖南中医药大学研究生院,湖南长沙410208

出　　处：《中国中医药现代远程教育》2020年第22期104-106,共3页Chinese Medicine Modern Distance Education of China

基　　金：湖南省教育厅一般项目【No.16C1226】;湖南省中医药研究院重点项目【No.201802】。

摘　　要：目的探讨平肥2号方对肥胖大鼠血清瘦素及磷酸化下丘脑组织信号转导和转录激活因子3(p-STAT3)和细胞因子信号转导抑制因子3(SOCS3)表达的影响。方法肥胖大鼠模型采用高脂饮食诱导,造模成功后将大鼠随机分成模型组、中药组及奥利司他组,另设置空白组以普食喂养,每组各10只。中药组予以平肥2号方灌胃、奥利司他组以奥利司他灌胃,模型组和空白组以蒸馏水灌胃,给药6周。观察各组大鼠体质量变化,检测血清瘦素、p-STAT3和SOCS3的表达。结果与空白组比较,模型组大鼠体质量、血清瘦素及SOCS3蛋白表达水平均升高,而p-STAT3蛋白表达减少,提示肥胖模型大鼠存在瘦素抵抗;与模型组比较,中药组及奥利司他组大鼠体质量及血清瘦素明显降低(P<0.01);与模型组比较,中药组大鼠下丘脑p-STAT3、SOCS3蛋白表达均升高(P<0.05)。结论平肥2号方能有效减轻肥胖大鼠体质量,减少血清瘦素表达,其机制可能是通过升高p-STAT3、SOCS3蛋白的表达从而改善其瘦素抵抗,纠正机体代谢异常。Objective To observe the effects of Pingfei No.2 on serum leptin and phosphorylated hypothalamic signal transduction and transcriptional activator 3(p-STAT3)and cytokine signal transduction inhibitor 3(SOCS3)expression in obese rats.Methods Rats that were successfully modeled in a high-fat diet-induced obese rat model were randomly divided into a model group,a Chinese medicine group,and an orlistat group,and a blank group,with 10 in each group.After successful modeling,Chinese medicine group with Pingfei No.2,orlistat group with orlistat,the model group and the blank group were orally administered with equal amounts of distilled water for 6 weeks.The body weight of each group was compared,and the expression of serum leptin and p-STAT3 and SOCS3 were detected.Results Compared with the blank group,the body weight,serum leptin and SOCS3 of the model group were increased,while the expression of p-STAT3 protein was decreased,suggesting that leptin resistance was observed in obese model rats.Compared with the model group,the body weight and serum leptin of the Chinese medicine group and the orlistat group were decreased(P<0.01).Compared with the model group,the expression of p-STAT3 and SOCS3 protein in the hypothalamus of the Chinese medicine group was increased(P<0.05).Conclusion Pingfei No.2 recipe can effectively reduce the body mass of obese rats,reduce the expression of serum leptin,that the mechanism may be by increasing the expression of p-STAT3 and SOCS3 proteins,and thereby improving leptin resistance,correcting metabolic abnormalities.

关 键 词：肥胖 平肥2号方 瘦素 实验研究 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]