TGF-β1通过ERK1/2通路上调人肝星状细胞程序性死亡配体1的表达  

TGF-β1 promotes PD-L1 expression of hepatic stellate cells by ERK1/2 signaling pathway

作　　者：李培培[1] 鲍士祥 金帅 常伟[1] 路景涛[1,2] 魏伟 LI Pei-pei;BAO Shi-xiang;JIN Shuai;CHANG Wei;LU Jing-tao;WEI Wei(Institute of Clinical Pharmacology,Anhui Medical University,Hefei 230032,China;School of Life Sciences,Anhui Medical University,Hefei 230032,China)

机构地区：[1]安徽医科大学临床药理研究所,安徽合肥230032 [2]安徽医科大学生命科学学院,安徽合肥230032

出　　处：《中国药理学通报》2020年第12期1660-1665,共6页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No 1708085MH181);国家自然科学基金资助项目(No 81773105)。

摘　　要：目的探讨TGF-β1上调肝星状细胞LX-2表达程序性死亡配体1(PD-L1)的作用及机制。方法5、10、20μg·L^-1 TGF-β1与肝星状细胞LX-2共培养,流式细胞术和Western blot检测LX-2细胞PD-L1蛋白的表达,Western blot检测ERK1/2和p-ERK1/2蛋白的表达。结果5、10、20μg·L^-1TGF-β1刺激LX-2细胞48 h后,LX-2表面的PD-L1表达明显增加;10μg·L^-1 TGF-β1刺激LX-2细胞24、48、72 h后,LX-2表面的PD-L1表达明显增加;5、10、20μg·L^-1TGF-β1刺激LX-2细胞48 h后,LX-2细胞ERK1/2含量不变,LX-2细胞p-ERK1/2的表达增加,同时ERK1/2特异性阻断剂U0126下调TGF-β1增加的LX-2细胞p-ERK1/2和PD-L1表达。结论TGF-β1能够增加LX-2表面PD-L1表达,其机制可能与ERK1/2信号通路有关。Aim To investigate the effect of TGF-β1 on the induction of programmed death ligand^-1(PD-L1)expression in LX-2 and its mechanism.Methods 5,10 and 20μg·L^-1 TGF-β1 was added to LX-2 cells and incubated.Then the expression of PD-L1was detected by flow cytometry and Western blot,and the expression of ERK1/2,p-ERK1/2 was detected by Western blot.Results TGF-β1(5,10,20μg·L^-1)increased significantly the expression of PD-L1 on LX-2 cells stimulated for 48 h,and TGF-β1(10μg·L^-1)could also markedly increase the expression of PD-L1 on LX-2 cells stimulated for 24,48 and 72 h.TGF-β1(5,10,20μg·L^-1)could up-regulate the expression of p-ERK1/2 in LX-2 cells stimulated for 48 h,and the expression of ERK1/2 remained unchanged;meanwhile,U0126 down-regulated TGF-β1-increased PD-L1 and p-ERK1/2 expression on LX-2 cells.Conclusion TGF-β1 could obviously increase the expression of PD-L1 on LX-2 via p-ERK1/2 signaling pathway.

关 键 词：肝星状细胞 转化生长因子β1 T淋巴细胞 程序性死亡配体1 ERK1/2 P-ERK1/2 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]