地肤子粗提物对变异链球菌作用的体外实验研究  

The Experimental Study of the Effect of Extractive of Kochiae Scopariae on Streptococcus Mutans in vitro

作　　者：张梦驰 常琳[1] 涂方祥 卢友光[1] ZHANG Mengchi;CHANG Lin;TU Fangxiang;LU Youguang(Department of Preventive Dentistry,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China)

机构地区：[1]福建医科大学附属口腔医院预防科,福建医科大学口腔医学院,福州350002

出　　处：《福建医科大学学报》2020年第5期287-290,共4页Journal of Fujian Medical University

基　　金：福建省中医药科研项目(2017FJZYZY205)。

摘　　要：目的研究地肤子粗提物对变异链球菌生长、黏附、产酸的影响,为地肤子粗提物抑制龋齿的研究提供科学依据。方法用80%的乙醇通过浸渍法提取地肤子粗提物,采用抑菌圈法、黏附实验及产酸实验,研究地肤子粗提物对变异链球菌体外生长的影响。结果地肤子粗提物浓度在0.78~100 mg/mL时,各组均未出现抑菌圈;当浓度≥1.25 mg/mL时,与对照组比较,地肤子粗提物对变异链球菌黏附及产酸有明显抑制作用(P<0.001)。结论地肤子粗提物浓度为100 mg/mL时,对变异链球菌的体外生长无抑制作用;在1.25 mg/mL时,对变异链球菌的黏附、产酸有明显抑制作用。Objective To study the inhibitory effect of kochiae scopariae extractives on anti-carious performance,adherence,acid generation of streptococcus mutans in vitro.To provide scientific basis for natural medicine on caries prevention.Methods The extractives of kochiae scopariae was extracted by alcohol to study the inhibitory effect of crude extractives of kochiae scopariae at different concentrations on the growth,adhesion and acid production of streptococcus mutans in vitro.Results At the concentration of 100,50,25,12.5,6.25,3.13,1.56,0.78 mg/mL,the extractives of kochiae scopariae had no inhibitory effect on the growth of streptococcus mutans in vitro.Compared with the control group,it had an obvious inhibitory effect on the adherence and acid generation of streptococcus mutans when the concentration was no less than 1.25 mg/mL(P<0.001).Conclusion The extractive of kochiae scopariae at the concentration of 100 mg/mL has no inhibitory effect on the growth of streptococcus mutans in vitro.The extractive of kochiae scopariae has obvious inhibitory effect on the adherence and acid generation of streptococcus mutants.

关 键 词：地肤子 变异链球菌 生长 细菌黏附 

分 类 号：R378.12[医药卫生—病原生物学]