Dectin-1、CARD9基因在角质形成细胞对红色毛癣菌应答中的作用  

The roles of Dectin-1 and CARD9 genes in the response of HaCaT cells against Trichophyton rubrum

作　　者：陈佳沁 陈剑[1] 邓威威 苏真 梁盼盼[1] 湛舒婷 Chen Jiaqin;Chen Jian;Deng Weiwei;Su Zhen;Liang Panpan;Zhan Shuting(Department of Dermatology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院皮肤科,广州510630

出　　处：《新医学》2020年第12期911-914,共4页Journal of New Medicine

基　　金：广东省自然科学基金(2015A030313137)。

摘　　要：目的研究树突状细胞相关性C型凝集素-1(Dectin-1)和胱天蛋白酶募集域蛋白9(CARD9)基因在角质形成细胞对红色毛癣菌应答中的作用。方法将红色毛癣菌与正常HaCaT细胞、Dectin-1、CARD9基因敲除HaCaT细胞共培养,用ELISA分析其细胞上清液中IL-6、IL-8的表达情况。结果Dectin-1基因敲除导致HaCaT细胞IL-6和IL-8基础分泌明显增加,敲除CARD9基因导致HaCaT细胞IL-6基础分泌增加(P<0.05)。在红色毛癣菌的刺激下,和未感染相比,HaCaT细胞分泌的IL-8明显减少(P<0.05);Dectin-1基因敲除的HaCaT细胞则表现为IL-6、IL-8分泌量明显减少(P均<0.05);CARD9基因敲除的HaCaT细胞的IL-6、IL-8分泌则无明显变化(P均>0.05)。结论Dectin-1基因和CARD9基因可能参与了维持表皮免疫稳态,CARD9基因还可能介导了红色毛癣菌引起的免疫应答。Objective To investigate the roles of Dectin-1 and caspase recruitment domaincontaining protein 9(CARD9)genes in the response of HaCaT cells against Trichophyton rubrum.Methods In this experiment,Trichophyton rubrum was co-cultured with HaCaT cells,Dectin-1^-/-HaCaT cells and CARD9^-/-HaCaT cells,respectively.The expression levels of IL-6 and IL-8 in the cell supernatant were assessed by ELISA.Results Dectin-1 gene knockout resulted in a significant increase in the secretion of IL-6 and IL-8(both P<0.05),CARD9 gene knockout increased the secretion of IL-6 in HaCaT cells(P<0.05).After the infection of Trichophyton rubrum,the secretion of IL-8 was significantly decreased in HaCaT cells(P<0.05).Dectin-1^-/-HaCaT cells showed a significant decrease of IL-6 and IL-8(both P<0.05),whereas no evident change was observed in the secretion of IL-6 and IL-8 in CARD9^-/-HaCaT cells(both P>0.05).Conclusions Dectin-1 and CARD9 genes may play an important role in maintaining the immune homeostasis of the epidermis.CARD9 gene may be involved in mediating the immune response against Trichophyton rubrum.

关 键 词：红色毛癣菌 HACAT细胞 树突状细胞相关性C型凝集素-1 胱天蛋白酶募集域蛋白9 

分 类 号：R756[医药卫生—皮肤病学与性病学]