牛大力标准提取物制备工艺研究  

Study on the Preparation Process of Standard Extract in the Root of Millettia speciosa Champ

作　　者：勾玲[1,2] 唐珍 苏春蓝 姚水莲 卢海啸 GOU Ling;TANG Zhen;SU Chun-lan;YAO Shui-lian;LU Hai-xiao(Yulin Normal University,Yulin 537000,China;Guilin Tourism University,Guilin 541006,China)

机构地区：[1]玉林师范学院,广西玉林537000 [2]桂林旅游学院,广西桂林541006

出　　处：《海峡药学》2020年第11期1-3,共3页Strait Pharmaceutical Journal

基　　金：广西高校中青年教师科研基础能力提升项目(2019KY0824);广西区大学生创新创业训练项目(201811837016)。

摘　　要：目的优化牛大力标准提取物制备工艺。方法采用正交试验L 18(36)设计优化牛大力标准提取物制备工艺,采用高效液相色谱法测定提取物中芒柄花素含量。结果最佳制备工艺为细粉,20倍量90%乙醇80℃加热回流提取2次,1次1.5 h,测得芒柄花素含量为284μg·g-1。结论所建立的方法简便、高效、稳定,可作为牛大力标准提取物的提取方法推广运用。OBJECTIVE Optimization of preparation technology of standard extract of Millettia speciosa Champ.METHODS The L 18(36)orthogonal experiment was used to optimize the extraction process of Millettia speciosa Champ.The contents of formononetin was detected by HPLC.RESULTS The optimal condition for extraction were as follows:Fine powder of Millettia speciosa Champ was 80℃reflux extracted with 20 times 90%ethanol for 1.5 h,for two times.The contents of formononetin was 284μg·g-1.CONCLUSION The method is simple,efficient and stable,and it can be used as a standard extract extraction method for Millettia speciosa Champ.

关 键 词：牛大力 芒柄花素 高效液相色谱法 最佳提取工艺 

分 类 号：R94[医药卫生—药剂学]