土壤微生物对异噁草酮连续施用的响应  

The Response of Soil Microbial Community to Repeated Application Clomazone

作　　者：张盈[1,2,3] 吴小虎 李晓刚 段婷婷[1] 徐军[2] 董丰收[2] 刘新刚[2] 郑永权[2] ZHANG Ying;WU Xiao-hu;LI Xiao-gang;DUAN Ting-ting;XU Jun;DONG Feng-shou;LIU Xin-gang;ZHENG Yong-quan(Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,State Key Laboratory for Biology of Plant Diseases and Insect Pests,Ministry of Agriculture and Rural Affairs,Key Laboratory of Control of Biological Hazard Factors(Plant Origin)for Agri-product Quality and Safety,Ministry of Agriculture and Rural Affairs,Beijing 100193;College of Plant Protection,Hunan Agricultural University,Changsha 410128)

机构地区：[1]贵州省农业科学院植物保护研究所,贵阳550006 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室农业农村部农产品质量安全生物性危害因子(植物源)控制重点实验室,北京100193 [3]湖南农业大学植物保护学院,长沙410128

出　　处：《生物技术通报》2020年第12期64-74,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31672057)。

摘　　要：本实验研究了温室条件下,连续两年施用异噁草酮后土壤微生物群落组成及N转化功能微生物的响应。第二次施药后第7天、15天、30天、60天和90天采集土壤,测定异噁草酮残留量和相关微生物指标。研究结果显示异噁草酮降解半衰期为17.4 d。施药后第15天细菌拷贝数降低,Alpha多样性增加;真菌拷贝数在第15天和60天显著降低,Alpha多样性在第7天和第90天显著降低。主成分分析(PCoA)显示,整个培养期间细菌和真菌的群落组成显著改变。分子生态网络分析表明,异噁草酮增加分子生态网络中节点数、连接数、平均度和平均路径长度,显著改变模块枢纽与联络者。FAPROTAX功能预测结果表明异噁草酮主要降低反硝化作用。This work aims to investigate the responses of soil microbial communities and microbes with nitrogen-transforming functions to 2 years consecutive application of clomazone under greenhouse conditions.Soil was sampled after 7,15,30,60,and 90 d of second application and clomazone residue concentration and relevant microbial parameters were determined.The half-lives of clomazone in this experiment were 17.4 d.The results showed that copy numbers of bacteria decreased,whereas bacterial Alpha diversity increased.Copy numbers of fungi(day 15 and day 60)and fungal diversity(day 7 and day 90)significantly decreased.Principal co-ordinates analysis(PCoA)results revealed that the bacterial and fungal community structure significantly changed during the whole culture period.Furthermore,molecular ecological network analysis indicated that the network of clomazone-treated soils contained more nodes,links,higher average degree and average path distance than control soils,i.e.,clomazone application led to remarkable changes in key species of its community network.FAPROTAX functional prediction results showed clomazone mainly reduced denitrification function.

关 键 词：异噁草酮 土壤微生物 群落结构 分子生态学网络 功能预测 

分 类 号：S482.4[农业科学—农药学] S154.3[农业科学—植物保护]