短串联靶标模拟技术及其在植物miRNA功能研究中的应用  

Short Tandem Target Mimic and Its Application in Analyzing Plant miRNA Functions

作　　者：郑文清 张倩[1] 杜亮 ZHENG Wen-qing;ZHANG Qian;DU Liang(College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083)

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《生物技术通报》2020年第12期256-264,共9页Biotechnology Bulletin

基　　金：北京林业大学科技创新计划项目(BLX2014-18);国家自然科学基金项目(31501168)。

摘　　要：MicroRNA(miRNA)作为一类大小长约21-24个核苷酸的内源非编码RNA,通过影响靶基因mRNA的稳定性或者翻译过程调控基因的表达。随着现代分子生物学技术的发展,越来越多的研究表明miRNA在植物的生长和发育过程中发挥着重要的作用。但是,miRNA功能研究缺乏有效的、适用性广的方法。介绍了一种可以特异地靶标miRNA方法,即短串联靶标模拟(Short tandem target mimic,STTM),并对STTM的基本特点和作用机制、与其他类似技术的比较以及其在植物中miRNA功能研究中的应用等方面进行了总结,有望为今后植物miRNA的功能研究提供技术参考。MicroRNA(miRNA),a class of endogenous non-coding RNAs in about 21-24 nucleotides length,regulates gene expression by affecting the stability of target mRNA or by regulating the process of translation.With the development of modern molecular biology technology,more and more studies demonstrated that miRNAs play an important role in the growth and development of plants.However,in the miRNA function research,more effective and widely applicable methods are needed.Here,a specific miRNA targeting method is introduced,namely Short Tandem Target Mimic(STTM).The basic characteristics,function mechanism of STTM,comparison with other similar technologies,and its application in the study of miRNA function in plants are described,which may provide a technical reference for the study of plant miRNA function in the future.

关 键 词：植物 短串联靶标模拟 MIRNA 调控机制 

分 类 号：Q943.2[生物学—植物学]