2013-2015年江苏省腮腺炎病毒基因特征和G基因型病毒株全基因序列分析  

Genetic characteristics of mump viruses and whole genome sequences of G genotype strains in Jiangsu province,2013-2015

作　　者：邓秀英[1] 胡莹[1] 陆培善[1] 郭宏雄[1] Deng Xiuying;Hu Ying;Lu Peishan;Guo Hongxiong(Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,Jiangsu,China)

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009

出　　处：《中国疫苗和免疫》2020年第5期534-539,共6页Chinese Journal of Vaccines and Immunization

摘　　要：目的分析江苏省腮腺炎病毒(Mumps virus,MuV)流行株SH基因特征和G基因型MuV全基因序列特征。方法对江苏省2013-2015年流行性腮腺炎病例的咽拭子标本进行MuV分离和基因分型,分析F和G基因型MuV的SH基因特征;对G基因型MuV进行全基因序列测定和拼接,重点分析F、HN和N蛋白基因特征。结果共获得2013-2015年178株F基因型和6株G基因型MuV流行株。SH基因亲缘关系树显示,F、G基因型流行株与其参考株之间核苷酸遗传距离分别为0.048-0.010、0.032-0.054,且G基因型形成两个独立分支。全基因序列分析显示,G基因型MuV的HN蛋白出现N-糖基化位点增加或减少,发生HN蛋白E356D、Y442S、I279T和I287V变异、N蛋白L457A、T466N、M492I和N495H变异以及F蛋白K101R变异。结论江苏省2013-2015年MuV流行株以F基因型为主;并发现G基因型MuV且发生一定变异。Objective To analyze genetic characteristics of mumps virus(MuV)and whole genome sequences of G genotype strains in Jiangsu province.Methods We isolated MuV strains from throat swab samples from mumps cases during 2013-2015 in Jiangsu,determined MuV genotypes,and analyzed characteristics of SH genes of F and G genotype isolates.We sequenced and spliced whole genomes of G genotype isolates to determine genetic characteristics of F,HN and N genes.Results We obtained 178 F genotype and 6 G genotype MuV strains during 2013-2015 in Jiangsu.Phylogenetic analysis for SH genes showed that the nucleotide genetic distances of F and G genotype isolates were 0.048-0.010 and 0.032-0.054 when compared with reference strains;the G genotype isolates formed two independent branches.Whole gene sequence analysis showed that N-glycosylation sites in the HN protein increased or decreased,with HN protein mutations of E356 D,Y442 S,I279 T,and I287 V;with N protein mutations of L457 A,T466 N,M492 I,and N495 H;and with an F protein mutation of K101 R.Conclusion The predominant MuV strain circulating in Jiangsu was F genotype;genotype G strains were detected and had genetic variation.

关 键 词：腮腺炎病毒 F基因型 G基因型 全基因组 基因特征 

分 类 号：R186[医药卫生—流行病学]