岩黄连与抗生素配伍后对金黄色葡萄球菌体外抑菌效果的比较  

BACTERIOSTASIS OF CORYDALIS SAXICOLA COMBINED WITHANTIBIOTICS ON STAPHYLOCOCCUS AUREUS IN VITRO

作　　者：孙桃桃 蒋平 钟枝梅 方妍 杨雨晴 单浩浩 左瑞华[1,2] SUN Tao-tao;JIANG Ping;ZHONG Zhi-mei;FANG Yan;YANG Yu-qing;SHAN Hao-hao;ZUO Rui-hua(College of Biological and Pharmaceutical Engineering,West Anhui University,Liu’an,Anhui 237012,China;The Center of Engineering and Technology of the Health Breeding and the Animal Disease Surveillance and Early Warning,West Anhui University,Liu’an,Anhui 237012,China)

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]安徽省中药资源保护与持续利用工程实验室,安徽六安237012

出　　处：《井冈山大学学报（自然科学版）》2020年第6期103-106,共4页Journal of Jinggangshan University (Natural Science)

基　　金：国家中药材产业技术体系(CARS-21);安徽省本科质量工程项目(2016sjjd079,2018zygc075,2018jyxm1153,2018jyxm1155);皖西学院教学研究项目(wxxy2018026,wxxy2017078);皖西学院林下种草养鹅生态养殖模式与示范项目(WXZR201743);国家大学生创新创业训练项目(201810376058);安徽省地方科技创新项目-科技惠民示范工程(202007d07050003);安徽省大别山等革命老区和贫困县专项(201904f06020008);皖西学院自然科学重点项目(WXZR201932);皖西学院研究性学习项目wxxyx2019140。

摘　　要：目的为了探讨岩黄连与抗生素配伍后体外联合抑菌效果。方法使用岩黄连、青霉素、左氧氟沙星、磷霉素、头孢拉定作为试验药物,以金黄色葡萄球菌作为试验菌株,采用倍比稀释法测定岩黄连与四种常用抗生素的最小抑菌浓度,采用棋盘稀释法测定岩黄连与四种抗生素的联合抑菌浓度。结果岩黄连、青霉素、左氧氟沙星、磷霉素和头孢拉定最小抑菌浓度分别为20 mg/mL、0.25μg/mL、0.5μg/mL、0.5μg/mL和1μg/mL;岩黄连与青霉素、头孢拉定、左氧氟沙星联用后表现为协同作用;岩黄连与磷霉素联用后表现为无关作用。结论岩黄连与青霉素、头孢拉定、左氧氟沙星联用可达到高效抑制金黄色葡萄的作用,对临床应用具有一定的参考意义。Objective:To investigate the bacteriostasis effect of Corydalis saxicola combined with antibiotics in vitro.Methods:C.saxicola Bunting(CSB),penicillin,levofloxacin,fosfomycin sodium and cefradine were selected as investigational drugs,and Staphylococcus aureus was selected as investigational strains,and then we used the method of double dilution to detect the minimal inhibitory concentration of CBS and four drugs,and the fractional inhibitory concentration of CBS was detected by chessboard dilution method in alliance with penicillin,cefradine,levofloxacin and fosfomycin sodium.Results:The minimum inhibitory concentrations of CBS, penicillin, levofloxacin, fosfomycin sodium and cefradine were 20 mg/mL, 0.25 μg/mL, 0.5 μg/mL, 0.5μg/mL and 1 μg/mL, respectively. The fractional inhibitory concentration of CBS showed the synergistic actioncombined with penicillin, cefradine or levofloxacin, and indifference action combined with fosfomycin sodium.Conclusion: The combination of CBS and penicillin, cefradine or levofloxacin can effectively inhibit S. aureuswhich has a reference for the clinical application.

关 键 词：岩黄连 抗生素 最小抑菌浓度 体外联合抑菌 金黄色葡萄球菌 

分 类 号：R285.5[医药卫生—中药学]