针刺带脉穴对腹型肥胖小鼠白色脂肪棕色化影响的研究  

Study on effect of acupuncture at Daimai(GB26)point on white adipose tissue browning in abdominal obesity mice

作　　者：于梦芸 王海英 梁翠梅[2] 胡慧[2] YU Mengyun;WANG Haiying;LIANG Cuimei;HU Hui(Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学研究生院,100029 [2]北京中医药大学东方医院针灸科 [3]北京中医药大学东直门医院心血管科

出　　处：《环球中医药》2020年第12期2005-2010,共6页Global Traditional Chinese Medicine

基　　金：北京市科技计划课题“首都临床特色应用研究”专项(Z161100000516057)。

摘　　要：目的观察针刺对腹型肥胖小鼠体重和腹围及内脏脂肪组织中解耦联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖物活化受体γ共激活因子1α(peroxisome proliferators-activated receptorγcoactivator-1α,PGC-1α)以及锌指蛋白16(PR domain containing 16,PRDM16)基因和蛋白的表达的影响。方法雄性C57BL/6J小鼠随机分为空白组6只、造模组29只,以高脂饲料喂养建立腹型肥胖模型;将造模成功的12只小鼠随机分为模型组6只和针刺组6只,继续以高脂饲料喂养。将针刺组小鼠置于固定器内固定,针刺双侧“带脉”穴,深度3~4 mm,接电针,疏密波(2 Hz/15 Hz,1.5 mA),隔日1次,15分钟/次。模型组和空白组同步固定,共干预8周。测量小鼠腹围和体重,HE染色观察双侧肾周白色脂肪组织(white adipose tissue,WAT)和肩胛间棕色脂肪组织(brown adipose tissue,BAT)形态,荧光定量PCR法和Western Blot法检测WAT中UCP1、PGC-1α和PRDM16基因和蛋白的表达。结果(1)腹围与体重比较:干预前,与空白组相比,模型组的体重和腹围明显增加(P<0.05)。干预后,与空白组比较,模型组体重和腹围显著增加(P<0.05);与模型组相比,针刺组的体重和腹围明显下降(P<0.05)。(2)WAT和BAT病理形态比较:模型组WAT与空白组相比细胞体积明显增大,呈大空泡的脂肪室,白色脂肪特征明显;针刺组WAT细胞出现多室,细胞变小、变圆,组织更加致密;模型组小鼠BAT较空白组呈现出较大的、单个空泡脂肪室;针刺组细胞较小,细胞数目增多呈片状,细胞间的毛细血管更丰富。(3)WAT中UCP1、PGC-1α和PRDM16蛋白水平比较:与空白组相比,模型组的WAT中的UCP1、PGC-1α和PRDM16蛋白水平明显下降(P<0.05);与模型组相比,针刺组的UCP1和PRDM16蛋白水平明显升高(P<0.05)。(4)WAT中UCP1、PGC-1α和PRDM16 mRNA表达比较:与空白组相比,模型组WAT中UCP1、PGC-1α和PRDM16 mRNA表达显著下降(P<0.05);与模型组相比,针刺组WAT中UCP1和PRDM16 Objective To observe the effects of acupuncture on the protein and gene expression levels of uncoupling protein 1(UCP1),peroxisome proliferators-activated receptorγcoactivator-1α(PGC-1α)and PR domain containing 16(PRDM16)in the body weight,abdominal circumference and visceral adipose tissue of abdominal obesity mice.Methods Male C57BL/6J mice were randomly divided into 3 groups,6 mice in the blank group,29 mice in the modeling group,and the abdominal obesity mice model was established by feeding them with high fat diet.Then the 12 successful modeling mice were randomly divided into the model group and the acupuncture group,6 mice in each group,feeding them with high fat diet continuously.The mice in the acupuncture group were placed in the fixation,and the two sides of Daimai(GB26)points were pricked,with a depth of 3 to 4 mm,electroacupuncture,dilatation wave(2Hz/15Hz,1.5 mA),once every other day,for 15 min/time.The model group and the blank group were fixed synchronously for a total of 8 weeks.The abdominal circumference and body weight of mice were measured.The histopathological changes of white fat tissue(WAT)around the bilateral kidney and the brown adipose tissue(BAT)were observed by HE staining.The gene and protein expression levels of UCP1,PGC-1αand PRDM16 in WAT were detected by using quantitative real time PCR and Western Blot,separately.Results(1)Comparison of abdominal circumference and body weight:Before intervention,compared with the blank group,the body weight and abdominal circumference of the model group were significantly increased(P<0.05,P<0.05).After intervention,compared with the blank group,the body weight and abdominal circumference of the model group were significantly increased(P<0.05,P<0.05).compared with the model group,the abdominal circumference and body weight of the acupuncture group were significantly decreased(P<0.05,P<0.05).(2)Comparison of pathological morphology of WAT and BAT:Compared with the blank group,WAT in the model group showed significantly increased cell volume,la

关 键 词：针刺 腹型肥胖 带脉穴 白色脂肪 棕色脂肪 减肥 小鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]