褪黑素抑制玉米赤霉烯酮诱导的猪卵巢颗粒细胞凋亡  

Melatonin inhibits zearalenone-induced apoptosis of porcine ovarian granulosa cells

作　　者：周佳伟 吴俊静[1] 乔木[1] 王孝忠 刘贵生[1] 梅书棋[1] 彭先文[1] ZHOU Jia-wei;WU Jun-jing;QIAO Mu;WANG Xiao-zhong;LIU Gui-sheng;MEI Shu-qi;PENG Xian-wen(Hubei Key Laboratory of Animal Embryo and Molecular Breeding/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan430063,China;Animal Disease Prevention and Control Center of Yichang City,Yichang443000,Hubei,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉430064 [2]湖北省宜昌市动物疫病预防控制中心,湖北宜昌443000

出　　处：《湖北农业科学》2020年第22期151-154,共4页Hubei Agricultural Sciences

基　　金：湖北省技术创新专项重大项目(2019ABA084、2017ABA134);湖北省自然科学基金创新群体项目(2018CFA014);国家生猪产业技术体系项目(CARS-35)。

摘　　要：以原代培养的猪卵巢颗粒细胞为模型,探究玉米赤霉烯酮(ZEA)对猪卵巢颗粒细胞凋亡的影响,并检测褪黑素(MT)在调节ZEA对猪卵巢颗粒细胞凋亡中的作用。结果表明,3和10μmol/L的ZEA对猪卵巢颗粒凋亡的影响不显著,但30、50和100μmol/L的ZEA显著促进猪卵巢颗粒细胞的凋亡,且呈现剂量效应关系;0.1和1μmol/L的MT抑制30μmol/L的ZEA诱导猪卵巢颗粒细胞的凋亡,能够提高细胞活力。Primary cultured porcine ovarian granulosa cells were used as a model to explore the effect of ZEA on the apoptosis of porcine ovarian granulosa cells,and to determine whether melatonin(MT)regulated the effect of ZEA on the apoptosis of porcine ovarian granulosa cells.The results showed that 3 and 10μmol/L ZEA had no significant effect on the apoptosis of porcine ovarian granulosa cells,but 30,50 and 100μmol/L ZEA significantly promoted the apoptosis of porcine ovarian granulosa cells,and showed a dose-effect relationship;It was found that 0.1 and 1μmol/L MT partially inhibited 30μmol/L ZEA-induced apoptosis of porcine ovarian granulosa cells and increased cell viability.

关 键 词：玉米赤霉烯酮 褪黑素 猪卵巢颗粒细胞 细胞凋亡 

分 类 号：S858.28[农业科学—临床兽医学]