不同外源酶组合对棕榈粕原料体外养分消化率和酶水解物能值的影响  

Effects of Different Combinations of Exogenous Enzymes on in Vitro Nutrient Digestibility and Enzymatic Hydrolyzate Gross Energy of Palm Kernel Meal Ingredients

作　　者：张顺芬 钟儒清 张碧峰 李滔 孙曙东 陈亮[1] 张宏福[1] ZHANG Shunfen;ZHONG Ruqing;ZHANG Bifeng;LI Tao;SUN Shudong;CHEN Liang;ZHANG Hongfu(State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Hunan United-Hope Biotechnology Company,Changsha 410007,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 [2]湖南友联禾邦生物技术有限公司,长沙410007

出　　处：《动物营养学报》2020年第12期5916-5924,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中央级公益性科研院所基本科研业务费专项(2020-YWF-ZX-04);友联禾邦横向项目(2017-SY-06);中国农业科学院科技创新工程(ASTIP-IAS07)。

摘　　要：本试验旨在利用单胃动物仿生消化系统(SDS-Ⅱ)探究不同外源酶组合对棕榈粕原料体外干物质消化率(IVDMD)、体外粗蛋白质消化率(IVCPD)、体外能量消化率(IVGED)和酶水解物能值(EHGE)的影响,以评价不同来源棕榈粕[马来西亚棕榈粕(MPK)和菲律宾棕榈粕(PPK)]的营养价值并筛选对应的最优外源酶组合。试验1采用2×3因子设计,设6个组,即分别以不添加外源酶的PPK和MPK原料作为对照组,以及分别向PPK原料中添加酶包-A(EP-A)、酶包-B(EP-B)和分别向MPK原料中添加EP-A、EP-B作为试验组。利用SDS-Ⅱ模拟猪全消化道消化过程,测定各组的IVDMD、IVCPD、IVGED和EHGE。试验2采用单因素完全随机设计,设5个组(EP0、EP1、EP2、EP3和EP4组),以不添加外源酶的EP0组为对照组,试验组分别在MPK原料中添加不同的外源酶组合,分别测定各组的IVDMD、IVCPD、IVGED和EHGE,以筛选最优外源酶组合。2个试验中每个样品均设5个重复,每个重复设1根消化管。结果显示:1)PPK的IVDMD、IVCPD、IVGED均显著高于MPK(P<0.05),PPK的EHGE与MPK相比无显著差异(P>0.05)。2)添加外源酶组合EP-A和EP-B均能显著提高MPK和PPK的IVGED和EHGE(P<0.05),添加外源酶组合EP-A和EP-B均能显著提高MPK的IVCPD(P<0.05),添加外源酶组合EP-B能显著提高MPK和PPK的IVDMD(P<0.05)。3)添加不同外源酶组合均能显著提高MPK的IVDMD、IVCPD、IVGED和EHGE(P<0.05),且EP4组的IVDMD、IVGED和EHGE显著高于EP1、EP2和EP3组(P<0.05)。由此可见,PPK的IVDMD、IVCPD、IVGED均高于MPK,为更加优质的棕榈粕来源;添加外源酶组合EP-A和EP-B均可提高2种来源棕榈粕的IVDMD、IVCPD、IVGED和EHGE,且EP-B效果更佳;EP4组的外源酶组合为酶解MPK的最优组合。This experiment was conducted to investigate the effects of different combinations of exogenous enzymes on in vitro dry matter digestibility(IVDMD),in vitro crude protein digestibility(IVCPD),in vitro gross energy digestibility(IVGED)and enzymatic hydrolyzate gross energy(EHGE)of palm kernel meal ingredients using a computer-controlled simulated digestion system(SDS-Ⅱ),and to evaluate the nutritional value of palm kernel meal from two sources[Malaysia palm kernel cake(MPK)and Philippines palm kernel cake(PPK)]and screen the corresponding optimal exogenous enzyme combination.In experiment 1,a 2×3 factor design was adopted,consisted of 6 groups,in which the control groups were MPK and PPK ingredients without exogenous enzymes addition,and the experimental groups were supplemented exogenous enzyme package-A(EP-A),exogenous enzyme package-B(EP-B)in PPK ingredient and supplemented EP-A,EP-B in MPK ingredient,respectively.Using SDS-Ⅱto simulate the total tract digestive process of pigs,the IVDMD,IVCPD,IVGED and EHGE of each groups were determined.In experiment 2,a single factor completely random design adopted,consisted of 5 groups(EP0,EP1,EP2,EP3 and EP4 groups),in which the control group(EP0 group)were without exogenous enzymes addition,and the experimental groups were supplemented different combinations of exogenous enzymes in MPK ingredient.The IVDMD,IVCPD,IVGED and EHGE of each groups were determined.Five replicates per sample and one digestive tract for each replicate were set in both experiments.The results showed as follows:1)the IVDMD,IVCPD and IVGED of PPK were significantly higher than those of MPK(P<0.05),but the EHGE of PPK had no significant difference with that of MPK(P>0.05).2)The supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVGED and EHGE of MPK and PPK(P<0.05),the supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVCPD of MPK(P<0.05),and the supplementation of exogenous enzyme combination EP-B significanltly

关 键 词：仿生消化法 棕榈粕 外源酶组合 酶水解物能值 

分 类 号：S816[农业科学—饲料科学]