银屑病患者皮损间充质干细胞的培养鉴定及其表达HES1和CXCL6的研究  

Culture and identification of dermal mesenchymal stem cells from skin lesions of patients with psoriasis and analysis of HES1 and CXCL6 expression in these cells

作　　者：邢建晓 刘燕敏 李小芳 李娇[1] 侯慧 李娟[1] 李俊琴[1] 张开明[1] Xing Jianxiao;Liu Yanmin;Li Xiaofang;Li Jiao;Hou Hui;Li Juan;Li Junqin;Zhang Kaiming(Department of Dermatology,Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis,Taiyuan Central Hospital of Shanxi Medical University,Shanxi 030009,China)

机构地区：[1]山西医科大学附属太原市中心医院,免疫性皮肤病干细胞山西省重点实验室,030009

出　　处：《中华皮肤科杂志》2020年第12期1004-1007,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金(81472888、81602768、81803146)。

摘　　要：目的培养鉴定银屑病患者皮损处真皮间充质干细胞(DMSC),并研究DMSC的发状分裂相关增强子1(HES1)和趋化因子配体6(CXCL6)表达情况。方法分离培养15例银屑病患者皮损及18例健康人(健康对照组)皮肤的DMSC,利用流式细胞仪进行表型鉴定,实时荧光定量聚合酶链反应(RT-PCR)及免疫印迹(Western blot)检测HES1和CXCL6的mRNA及蛋白表达水平,两组间比较采用t检验。结果银屑病组DMSC形态与健康对照组无差异,但HES1和CXCL6基因的mRNA水平分别是对照组的3.56和3.44倍,且差异有统计学意义(P<0.05)。在蛋白水平,银屑病组DMSC中HES1和CXCL6的表达明显高于对照组(P<0.05)。结论银屑病患者皮肤DMSC HES1及CXCL6表达升高可能参与银屑病的发病。Objective To culture and identify dermal mesenchymal stem cells(DMSCs)in skin lesions of patients with psoriasis,and to determine the expression of hairy and enhancer of split-1(HES1)and chemokine ligand 6(CXCL6)in DMSCs.Methods DMSCs were isolated from skin lesions of 15 patients with psoriasis and normal skin tissues of 18 healthy controls,and then subjected to culture.Cell phenotypes were identified by flow cytometry,and mRNA and protein expression of HES1 and CXCL6 was determined by real-time fluorescence-based quantitative PCR(RT-PCR)and Western blot analysis respectively.Comparisons were performed between 2 groups by using t test.Results There was no difference in the morphology of DMSCs between the psoriasis group and control group.The mRNA expression of HES1 and CXCL6 in the psoriasis group was 3.56 and 3.44 times that in the control group respectively,and there was a significant difference between the two groups(both P<0.05).The protein expression of HES1 and CXCL6 in DMSCs was significantly higher in the psoriasis group than in the control group(both P<0.05).Conclusion The high expression of HES1 and CXCL6 in DMSCs from lesions may be involved in the occurrence of psoriasis.

关 键 词：银屑病 间质干细胞 趋化因子 CC 发状分裂相关增强子1 趋化因子配体6 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]