白花败酱草乙醇部位化学成分研究  

Chemical constituents from ethanol extract of Patrinia villosa Juss

作　　者：刘伟 刘洋成[1] 王嘉琪 陈长兰[1] 项峥 LIU Wei;LIU Yang-cheng;WANG Jia-qi;CHEN Chang-lan;XIANG Zheng(School of Pharmaceutical Science,Liaoning University,Shenyang 110036,China)

机构地区：[1]辽宁大学药学院,沈阳110036

出　　处：《哈尔滨商业大学学报（自然科学版）》2020年第6期660-664,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金(81303211);中国博士后科学基金(2013M540301);沈阳市高层次创新人才计划—中青年创新支持计划(RC190105)。

摘　　要：研究了白花败酱草的化学成分,采用70%乙醇提取白花败酱草,以石油醚、二氯甲烷、正丁醇萃取提取物得到不同极性的萃取物.取二氯甲烷层萃取物依次经正相硅胶柱色谱、ODS柱色谱、葡聚糖凝胶色谱进行分离,应用半制备高效液相色谱进行进一步的纯化提取.将得到的单体化合物通过核磁共振技术得到结构数据,解析并与相关文献比对后确定化合物结构.共分离得到了5个单体化学成分,分别为Caffeic acid methyl ester、Caffeic acid ethyl ester、Methyl 2-(4-hydroxyphenyl)acetate、(E)-Ferulic acid、Cirsimaritin.其中1个化合物为首次从败酱属植物中分离得到.In this experiment,the chemical constituents of Patrinia villosa Juss.were studied.Patrinia villosa Juss.was extracted by 70%ethanol,and different polar extracts were obtained by petroleum ether,dichloromethane and n-butanol.The extractions of dichloromethane were sequentially separated by normal silica gel column chromatography,octadecyl silane(ODS)column chromatography and sephadex chromatography.The semi-preparative high performance liquid chromatography was used for further purification and extraction.The structure data of monomers were obtained by the nuclear magnetic resonance,and the structures of the compounds were determined after analysis and comparison with the relevant literature.Five compounds were isolated,including caffeic acid methyl ester,caffeic acid ethyl ester,methyl 2-(4-hydroxyphenyl)acetate,(E)-ferulic acid,and cirsimaritin.One compound was isolated from patrinia for the first time.

关 键 词：白花败酱草 化学成分 分离 核磁共振 结构鉴定 

分 类 号：R931.6[医药卫生—生药学]