阻断Omi/HtrA2对大鼠骨骼肌细胞LC3的影响  

Blocking the Effect of Omi/HtrA2 on LC3 of Rat Skeletal Muscle Cells

作　　者：张欣 郭威 ZHANG Xin;GUO Wei(School of Sports and Health Sciences,Nanjing Institute of Physical Education,Nanjing 210014,China;Jinling Institute of Technology,Nanjing 211169,China)

机构地区：[1]南京体育学院运动健康科学学院,江苏南京210014 [2]金陵科技学院,江苏南京211169

出　　处：《南京体育学院学报》2020年第10期55-57,共3页Journal of Nanjing Sports Institute

基　　金：南京体育学院国家级培育项目(PY201921)。

摘　　要：目的:通过阻断Omi/HtrA2,探究Omi/HtrA2对大鼠骨骼肌LC3的影响,明确Omi/HtrA2在骨骼肌自噬中的作用。方法:8周龄雄性大鼠88只随机分为对照组(C,n=8)、安慰剂组(D,n=40)、抑制剂组(U,n=40)。安慰剂组注射相对量的DMSO溶液,抑制剂组注射Omi/HtrA2特异性抑制剂Ucf-1。干预后0h、12h、24h、48h和72h取材。免疫荧光技术观测LC3位置及表达量;Western Blot检测比目鱼肌LC3II/I的蛋白表达。结果:大鼠骨骼肌细胞LC3荧光强度量,D组与C组无显著性差异,阻断Omi/HtrA2后出现一过性降低,其中12 h至24 h时间点降至最低;LC3II/I的蛋白表达出现相同趋势。结论:(1)阻断Omi/HtrA2可抑制LC3II/I表达;(2)12 h至24 h是Omi/HtrA2阻断剂发挥作用的关键时间段。Objective:In this study,the effects of Omi/HtrA2 on LC3 in skeletal muscle of rats were investigated by blocking Omi/HtrA2,so as to clarify the role of Omi/HtrA2 in skeletal muscle autophagy.Methods:88 male rats aged 8 weeks were randomly divided into control group(C,n=8),DMSO group(D,n=40)and ucf-1 group(U,n=40).The placebo group was injected with a relative amount of DMSO solution,while the inhibitor group was injected with Omi/HtrA2 specific inhibitor ucf-1.After intervention,samples were harvested at 0h,12h,24h,48h and 72h.The position and expression of LC3 were observed by immunofluorescence technique.The protein expression of LC3II/I in soleus muscle was detected by Western Blot.Results:LC3 fluorescence intensity of rat skeletal muscle cells showed no significant difference between group D and Group C.Transient decrease occurred after blocking Omi/HtrA2,which was at the lowest point from 12h to 24h.The protein expression of LC3II/I showed the same trend.Conclusion:(1)blocking Omi/HtrA2 can inhibit the expression of LC3II/I;(2)12h to 24h is the key period for Omi/HtrA2 blockers to function.

关 键 词：OMI/HTRA2 骨骼肌细胞 LC3 

分 类 号：G804.2[文化科学—运动人体科学]