襄阳市育龄妇女血清B19病毒DNA的检测  

Detection of parvovirus B19 viral DNA in serum of women of childbearing age in Xiangyang City

作　　者：王艺静 江华[2] 张曦 郭冲 王媛 WANG Yijing;JIANG Hua;ZHANG Xi;GUO Chong;WANG Yuan(MedicalCollege of Art and Sicence of Hubei University,Xiangyang,Hubei 441053,China;Xiangyang Central Hospital,Xiangyang,Hubei 444102,China)

机构地区：[1]湖北文理学院医学院,湖北襄阳441053 [2]湖北省襄阳市中心医院

出　　处：《公共卫生与预防医学》2020年第6期91-95,共5页Journal of Public Health and Preventive Medicine

基　　金：襄阳市科技研究与开发项目(襄科计)(2017)32号;湖北省自然科学基金面上项目(2017CFB241);湖北文理学院博士科研启动经费资助;校级大学生创新创业训练计划项目(201810519092,X201910519064)。

摘　　要：目的分析襄阳市育龄妇女中B19病毒(Human Parvovirus B19)的感染情况,为该地区育龄妇女孕期保健提供参考。方法以2018—2019年襄阳市303例育龄妇女为研究对象,通过巢式PCR技术对其血清中的B19病毒DNA进行检测,比较正常妊娠、异常妊娠和不孕症样本中B19病毒DNA的检出率的差异,同时比较不同年龄阶段育龄妇女B19病毒DNA检出率的差异。结果全部303例育龄妇女的B19病毒DNA检出率为27.72%,其中26~35岁年龄阶段的育龄妇女病毒DNA检出率高于其他年龄组;同时异常妊娠组的B19病毒DNA检出比例显著高于正常妊娠组(P<0.05)。结论襄阳地区育龄妇女人群中异常妊娠和不孕症组的B19病毒DNA检出率显著高于正常妊娠组,并且在26~35岁年龄阶段的育龄妇女检出率较高,因此,需要加强该地区育龄妇女的孕期B19病毒筛查,降低其对胎儿流产等方面的影响。Objective To analyze the infection of human parvovirus B19 among women of childbearing age in Xiangyang City,and to provide a reference for pregnant women′s health care.Methods A total of 303 women of childbearing age in Xiangyang City from 2018 to 2019 were selected as the research subjects.B19 virus DNA in serum of the subjects was detected by nested PCR technology.The differences in the detection rate of B19 viral DNA among normal pregnancy,abnormal pregnancy,and infertility serum were statistically analyzed.The differences in the detection rate of B19 virus DNA among women of childbearing age at different ages were compared.Results The detection rate of B19 viral DNA in all 303 women of child-bearing age was 27.72%.The detection rate of B19 virus DNA in 26-35 year old women was higher than that in other age groups.The detection rate of B19 virus DNA in abnormal pregnancy group was significantly higher than that in normal pregnancy group(P<0.05).Conclusion The detection rate of B19 virus DNA in abnormal pregnancy and infertility group was significantly higher than that in normal pregnancy group,with the detection rate of B19 virus in 26-35 year old women of childbearing age being the highest among all age groups.It is necessary to strengthen the screening of B19 virus in pregnant women of childbearing age in this region to reduce its impact on fetal abortion.

关 键 词：育龄妇女 B19病毒DNA 巢式PCR 异常妊娠 

分 类 号：R173[医药卫生—妇幼卫生保健]