富铜微生物的筛选以及其培养条件优化  

Screening of copper rich microorganisms and optimization of culture conditions

作　　者：鲁陈[1] 刁欢[2] 丁小玲[1] 代张超 闫一博 李吕木[1] LU Chen;DIAO Huan;DING Xiaoling;DAI Zhangchao;YAN Yibo;LI Lvmu(School of Animal Science and Technology,Anhui Agriculture University,Hefei,Anhui Province 230036,China;Anhui Xinhan Phammacy College,Hefei,Anhui Province 230081,China)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽新华学院药学院,安徽合肥230081

出　　处：《中国饲料》2020年第23期20-25,共6页China Feed

基　　金：安徽省2018年重点研究与开发项目(1804g07020169);安徽省2019年高校青年人才项目(gxyq2019131)。

摘　　要：为分离高效富铜菌来转化无机铜为微生物铜,本试验以微生物富集转化无机铜为微生物铜的转化率为筛选指标,通过火焰原子吸收分光光度法测定胞内铜含量,试验从高铜土壤中通过稀释涂布平板法分离筛选得到一株无机铜高效转化真菌FT7,并进行26S rDNA鉴定,采用L9(34)正交设计对其温度、pH、接种量和培养时间4因素进行优化。结果表明:真菌FT7为黑曲霉(Aspergillus niger),其最佳富集转化条件为温度29.5℃、pH 5、接种量4%、时间5.5 d,其最高转化率为87.65%。综上,黑曲霉为饲用微生物,其转化无机铜得到的微生物铜可用于饲料中新型饲料添加剂。A high efficient copper rich bacteria was isolated to convert inorganic copper to microbial copper.The microbial transformation of inorganic copper enrichment microbial transformation rate of copper as an indicator,the flame atomic absorption determination of copper content in cell spectrophotometry test by plate dilution method from high copper in the soil was isolated from a strain of inorganic copper efficient conversion of fungus FT7,and 26S rDNA were identified,the L9(34)orthogonal design of the temperature,pH,temperature,inoculation and incubation time 4 factors were optimized.The results showed that fungus FT7 was Aspergillus niger,and its best enrichment and transformation conditions were temperature 29.5℃,pH 5,inoculation amount 4%and time 5.5 d,and the highest conversion rate was 87.65%.Aspergillus niger was a feeding microorganism,and the microbial copper obtained from the conversion of inorganic copper can be used as a new feed additive in the feed.

关 键 词：筛选 黑曲霉 微生物铜 转化率 

分 类 号：S816.3[农业科学—饲料科学]