甘草颗粒中甘草酸含量HPLC测定方法的建立  

Establishment of HPLC Method for Detecting Glycyrrhizic Acid Content in Glycyrrhiza Granules

作　　者：李军[1] 杨奇 刘立轩[1] 李敏 冷晓红[1] 马春芳 吴春燕 LI Jun;YANG Qi;LIU Li-xuan;LI Min;LENG Xiao-hong;MA Chun-fang;WU Chun-yan(Ningxia Vocational and Technical College,Ningxia Research Center for the Development and Utilization of Chinese Herbs,Yinchuan,Ningxia,750021,China;Ningxia Veterinary Drugs and Feed Administration,Yinchuan,Ningxia,750011,China)

机构地区：[1]宁夏职业技术学院宁夏中药材开发与利用工程技术研究中心,宁夏银川750021 [2]宁夏兽药饲料监察所,宁夏银川750011

出　　处：《动物医学进展》2020年第12期74-79,共6页Progress In Veterinary Medicine

基　　金：2018年农业行业(国家)标准项目兽药国家标准制修订计划(2018Z018)。

摘　　要：为完善甘草颗粒的质量标准,以C18(4.6 mm×250 mm,5μm)柱为色谱柱,甲醇-0.2 mol/L醋酸铵-冰醋酸(56∶44∶0.4)为流动相,流速1.0 mL/min,检测波长为250 nm,进样量为10μL的色谱条件建立了HPLC法测定甘草酸含量,并进行方法学验证。结果表明,甘草酸色谱峰峰形良好,且与相邻峰良好分离;进样浓度在0.1 mg/mL^0.5 mg/mL(0.1μg^0.5μg)范围内,与峰面积呈良好的线性关系(r=0.9998);精密度和稳定性等考察结果的相对标准偏差均小于2.0%;样品平均回收率为97.92%(n=6),RSD为1.87%。建立的方法可靠,准确度高,重现性好,为完善甘草颗粒的含量测定方法提供了依据。To improve the quality standard of Glycyrrhiza granules,the chromatographic conditions were as follow:C18 column(4.6 mm×250 mm,5μm)was used as the chromatographic column,methanol-0.2 mol/L ammonium acetate solution-glacial acetic acid(56∶44∶0.4)was as the mobile phase,the flow rate was 1.0 mL/min,the wavelength was 250 nm,and injection volume was 10μL.HPLC method for the content determination of glycyrrhizic acid was established and verified.The results showed that the chromatographic peak shape of glycyrrhizic acid could be separated completely.There was a good linear correlation between the peak areas and the concentrations of glycyrrhizic acid at the range of 0.1-0.5 mg/mL(R2=0.9998)by HPLC.The relative standard deviation(RSD)of precision and stability were less than 2.0%.The average recovery was 97.92%with RSD 1.87%(n=6).The results indicated that the method was reliable,accurate and repeatable,and provided basis for improving the content determination method of Glycyrrhiza granules.

关 键 词：甘草颗粒 甘草酸 高效液相色谱 含量测定 

分 类 号：S853.72[农业科学—临床兽医学] S859.2[农业科学—兽医学]