髓源性生长因子对糖尿病小鼠BMSCs骨向分化的影响及机制研究  

The effects of myeloid-derived growth factor on osteoblast differentiation of BMSCs from diabetic mice and the related mechanisms

作　　者：刘翠 徐晓丽 李金儒 朱彪 李欢[2] 向光大[2] 郭红延 LIU Cui;XU Xiaoli;LI Jinru;ZHU Biao;LI Huan;XIANG Guangda;GUO Hongyan(Department of Stomatology,Pristine Agency Clinic of Political Work Department of the Central Military Commission,100120 Beijing,China;Graduate School of Southern Medical University;Department of Stomatology,The PLA Hong Kong Garrison Hospital;Department of Stomatology,Affiliated Hospital of Hebei University;Department of Stomatology,The Third Medical Center,Chinese PLA General Hospital)

机构地区：[1]中央军委政治工作部原机关门诊部口腔科,北京100120 [2]南方医科大学研究生院 [3]中国人民解放军驻香港部队医院口腔科 [4]河北大学附属医院口腔科 [5]中国人民解放军总医院第三医学中心口腔科

出　　处：《实用口腔医学杂志》2020年第6期865-869,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81870573)。

摘　　要：目的:探讨髓源性生长因子(MYDGF)对糖尿病小鼠骨髓间充质干细胞(BMSCs)成骨分化的影响及其信号机制。方法:分离、培养糖尿病小鼠BMSCs,碱性磷酸酶(ALP)活性检测BMSCs的成骨活性,茜素红染色观察钙结节,实时荧光定量PCR(qPCR)检测成骨相关基因Runx2、Osx、ALP和OCN的表达,Western blot检测PI3K、Akt的磷酸化水平。结果:MYDGF呈浓度依赖性地增强ALP活性,促进钙结节形成,显著上调Runx2、Osx和ALP的表达(P<0.05),显著提高PI3K和Akt的磷酸化水平(P<0.05)。结论:MYDGF可通过激活PI3K/Akt信号通路促进糖尿病小鼠BMSCs骨向分化。Objective:To investigate the effects of myeloid-derived growth factor(MYDGF)on osteoblast differentiation of bone marrow mesenchymal stem cells(BMSCs)from diabetic mice and the related signaling mechanisms.Methods:BMSCs from diabetic mice were in vitro cultured in osteogenic differentiation medium,ALP activity was tested,qRT-PCR was used to evaluate the expression levels of Runx2,Osx,ALP and OCN,phosphorylation level of PI3K and Akt was detected by Western blot.Results:MYDGF promoted ALP activity in a concentration-dependent manner,increased mineralized nodules,significantly upregulated mRNA expression of Runx2,Osx and ALP(P<0.05);significantly increased phosphorylation level of PI3K and Akt(P<0.05).Conclusion:MYDGF promotes osteoblast differentiation of BMSCs from diabetic mice by activating PI3K/Akt signaling pathway.

关 键 词：髓源性生长因子(MYDGF) PI3K/AKT信号通路 骨髓间充质干细胞(BMSCs) 成骨细胞 

分 类 号：R587.1[医药卫生—内分泌] R329.2[医药卫生—内科学]